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Box of Hycult Biotech ELISA kit
Catalog # HK398

Transgelin, Human, Assay

The Human transgelin ELISA kit is to be used for the in vitro quantitative determination of transgelin in serum and plasma samples.

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Transgelin, known as SM22, emerges as a crucial 22 kDa calponin-like actin-binding protein that exhibits diverse isoforms (a-d) distinguished by their isoelectric points. This protein, soluble in water, predominates in intestinal smooth muscle tissues, underscoring its potential role in the maturation and differentiation of smooth muscle cells. As a marker, transgelin stands out in the detection of acute intestinal ischemia (AII), a severe abdominal emergency with a high risk of morbidity and mortality stemming from mesenteric blood flow disruption. This condition can lead to catastrophic damage to intestinal integrity, hypothesized to cause the release of muscle cell proteins like transgelin. Elevated transgelin levels in patients with transmural ischemia versus controls support this theory.

In the realm of oncology, transgelin’s diagnostic value becomes evident in leiomyosarcoma, a soft tissue sarcoma where myoid differentiation often points to a more aggressive disease profile. The correlation between transgelin and smooth muscle differentiation is significant, with studies revealing its abundance in leiomyosarcomatous differentiation. As researchers delve deeper into the nuances of transgelin’s function, its prominence in scientific investigations continues, with a focus on its biomarker potential for clinical emergencies and aggressive cancers.

Application
Immuno assays

Use
The Human transgelin ELISA kit is to be used for the in vitro quantitative determination of transgelin in serum and plasma samples.

Principle
The Human transgelin ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 1 hour and 15 minutes. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing Human transgelin. Peroxidase-conjugated antibody will bind to the captured transgelin. Peroxidase-conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the Human transgelin standards (log). The Human transgelin concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

Amount
1 x 96 det., 2 x 96 det.
Product type
Assays
Working volume
100 µl/well
Alias
22 kDa actin-binding protein, Protein WS3-10, sm22, SM22-alph, SMCC, Smooth muscle protein 22-alpha, TAGLN1, WS3-10
Gene
Gene Name: TAGLN Uniprot: Q01995 Entrez Gene ID: 6876
Species
Human
Disease
Gastroenterology
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Assay Manual
HK398
472 kb
Safety Data Sheet

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