CRAMP, Mouse, mAb AC6-DH2
The monoclonal antibody AC6-DH2 recognizes the mouse protein Cathelin-Related AntiMicrobial Peptide (Cramp). Cathelicidins are a familiy of antimicrobial proteins predominantly found in the peroxidase-negative granules of neutrophils. the cathelicidins are synthesized as preproteins. Within the neutrophils, they are stored in granules as inactive proforms after removal of the signal peptide. The biologic active domains of the cathelicidins reside in the C-terminus. The C-terminal antimicrobial peptides are activated when cleaved from the proforms of the cathelicidins by serine proteases from azurophilic granules. Cramp (Cathelin-Related Anti-Microbial Peptide) is the mouse analogue of human LL-37 peptide, which is the antibacterial C-terminus of hCAP-18 (human cathelicidin). CRAMP forms an amphipathic α-helix similar to other antimicrobial peptides. Cramp is a potent antibiotic against Gram-negative bacteria by inhibiting growth of a variety of bacterial strains and by permeabilizing the inner membrane of E.coli directly. Abundant expression of Cramp is found in myeloid precursors and neutrophils. Cramp represents the first antibiotic peptide found in cells of myeloid lineage in the mouse. Inflammatory cells in the mouse can thus use a non-oxidative mechanism for microbial killing.
|Product type||Monoclonal antibodies|
|Formulation||0.2 ml (100 μg/ml) 0.2 µm filtered in PBS+0.1%BSA|
|Application||Functional studies, Immuno assays|
|Immunogen||Full length mouse CRAMP peptide.|
|Alias||Cathelicidin antimicrobial peptide, Cathelin-like protein, CLP Gene name: Camp, Cnlp, Cramp Entrez Gene ID: 12796 Uniprot: P51437|
|Storage and stability||Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.|
|Precautions||For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.|
|Application:||Functional studies, Immuno assays|
|Application notes:||- IA: Antibody clone AC6-DH2 used as capture and detection antibody.
- FS: The bacterial activity of cramp peptide can be blocked with HM1125 in a dose dependent way.
- HM1125 cannot be used for Western blot.