NOD1, Human, mAb 2A10
Catalog #:
HM2290
The monoclonal antibody 2A10 recognizes human Nod1. The innate immune system is the first line of defense against pathogens using various pattern recognition receptors (PRR). PRRs include toll-like receptors (TLRs), nucleotide-biding oligomerization domain (NOD)-like receptors (NLRs) and retinoic acid-inducible (RIG)-I-like receptors (RLR). The Nod-like receptor (NLR) family of proteins consists of cytosolic pattern recognition molecules involved in sensing microbial and danger signals and triggering innate immune activation. The founding members of NLRs are Nod1 and Nod2. These intracellular receptors recognize peptidoglycans. The receptor consists of three main domains. The C-terminal site recognizes the ligand via the leucin-rich repeat domain (LRR). The NOD domain facilitates the selfoligomerization and contains the ATPase activity. The N-terminal site is the caspase-recruitement domain (CARD). Proteoglycans are polymers composed of glycan chains of alternating N-acylglucosamine (GlcNAc) and N-acetylmuramic acid (MurNAc) units cross-linked to each other by short peptides. The cross-linking of two parallel glycan chains is mediated by stem peptides that can be further linked by bridging amino acids. For activation of the immune system, Nod2 senses muramyl dipeptide (MDP), which is found in the PGN of nearly all Gram-positive and Gram-negative organisms, whereas Nod1 recognizes meso-diaminopimelic acid (meso-DAP)-containing PGN fragments. Stimulation of Nod1 or Nod2 results in the activation of NF-κB and MAPKs, through a homophilic interaction with the adaptor kinase, Rip2. Thereby driving the transcription of numerous genes involved in both innate and adaptive immune responses. Nod1 is widely expressed in many cell types and organs. Nod1 is associated with a susceptibility to inflammatory diseases, including asthma and atopic eczema and allergic diseases
| Product type | Monoclonal antibodies |
|---|---|
| Quantity | 100 µg |
| Formulation | 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide. |
| Application | Frozen sections, Immuno fluorescence, Immuno precipitation, Western blot |
| Use | For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting. |
| Immunogen | GST-tagged CARD domain (aa1–126) of Nod1 expressed in bacteria |
| Isotype | Rat IgG2a |
| Species | Human |
| Cross reactivity | Mouse - Weak |
| Alias | CARD4, Nucleotide-binding oligomerization domain-containing protein 1, Caspase recruitment domain-containing protein 4 |
| References | 1. Kufer, T et al; The pattern-recognition molecule Nod1 is localized at the plasma membrane at sites of bacterial interaction. Cell Microbiol 2008, 10: 477 2. Allison, C et al; Helicobacter pylori Induces MAPK Phosphorylation and AP-1 Activation via a NOD1-Dependent Mechanism. J Immunol 2009, 183: 8099 |
| Storage and stability | Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label. |
| Precautions | For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product |
| Disease | Infectious diseases |
| Application: | Frozen sections, Immuno fluorescence, Immuno precipitation, Western blot |
|---|---|
| Application notes: | W: A reduced sample treatment and SDS-Page was used. The band size is 110 kDa. |
| Use: | For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting. |
| References: | 1. Kufer, T et al; The pattern-recognition molecule Nod1 is localized at the plasma membrane at sites of bacterial interaction. Cell Microbiol 2008, 10: 477 2. Allison, C et al; Helicobacter pylori Induces MAPK Phosphorylation and AP-1 Activation via a NOD1-Dependent Mechanism. J Immunol 2009, 183: 8099 |
|---|
CoA-TDS HM2200 - 2300
Protocol
