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Catalog # HM2258

Complement factor D, Human, mAb D10/4


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The monoclonal antibody D10/4 recognizes human complement factor D. Factor D, a 27 kDa serine protease of the alternative complement pathway, is synthesized as a precursor single-chain molecule. The alternative complement pathway represents an important humoral component of natural defense against microbial attack. The participation of the alternative complement pathway has been implicated in the pathogenesis of a wide variety of human diseases.
Factor D is unique among serine proteases in that it requires neither enzymatic cleavage for expression of proteolytic activity nor activation by a serpin for its control. Factor D is highly specific and cleaves factor B bound to C3b, generating the C3bBb enzyme. Factor D is the rate-limiting C3 convertase enzyme of the alternative pathway. It has both the lowest plasma concentration and the lowest molecular weight of all complement components. Normal values in human EDTA plasma and urine are 1.05 (± 0.27) and 0.62 (± 0.33) µg/ml, respectively. In normal human subjects, factor D is rapidly eliminated via the kidney. Compared to controls, plasma and urine levels of factor D in patients with renal failure are elevated. Elevated plasma levels return to normal levels within seven days after successful renal transplantation to prevent overstimulation of the complement pathway.
Human adipsin, a protein that is expressed at high levels in adipose tissue, is identical to factor D and also displays the same enzymatic activity as factor D. This suggests that factor D plays a role in fatty tissue distinct from its role as a complement protein.

Immuno assays

For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions.

Product type
Monoclonal antibodies
100 µg, 20 µg
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Serum purified human factor D
Mouse IgG2a
C3 convertase activator, Properdin factor D, Adipsin
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hbt is not responsible for any patent infringements that might result from the use or derivation of this product.
Infectious diseases, Nephrology
Protocol CoA-TDS Safety Data Sheet

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