Home \ Antibodies \ Complement factor D, Human, mAb D10/4
Catalog # HM2258

Complement factor D, Human, mAb D10/4

120,00333,00

Please choose the quantity of items you wish to add to your cart…

The monoclonal antibody D10/4 recognizes human complement factor D. Factor D, a 27 kDa serine protease of the alternative complement pathway, is synthesized as a precursor single-chain molecule. The alternative complement pathway represents an important humoral component of natural defense against microbial attack. The participation of the alternative complement pathway has been implicated in the pathogenesis of a wide variety of human diseases.
Factor D is unique among serine proteases in that it requires neither enzymatic cleavage for expression of proteolytic activity nor activation by a serpin for its control. Factor D is highly specific and cleaves factor B bound to C3b, generating the C3bBb enzyme. Factor D is the rate-limiting C3 convertase enzyme of the alternative pathway. It has both the lowest plasma concentration and the lowest molecular weight of all complement components. Normal values in human EDTA plasma and urine are 1.05 (± 0.27) and 0.62 (± 0.33) µg/ml, respectively. In normal human subjects, factor D is rapidly eliminated via the kidney. Compared to controls, plasma and urine levels of factor D in patients with renal failure are elevated. Elevated plasma levels return to normal levels within seven days after successful renal transplantation to prevent overstimulation of the complement pathway.
Human adipsin, a protein that is expressed at high levels in adipose tissue, is identical to factor D and also displays the same enzymatic activity as factor D. This suggests that factor D plays a role in fatty tissue distinct from its role as a complement protein.

Application
Immuno assays

Use
For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions.

Product type
Monoclonal antibodies
Quantity
20 µg, 100 µg
Formulation
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Immunogen
Serum purified human factor D
Isotype
Mouse IgG2a
Species
Human
Alias
C3 convertase activator, Properdin factor D, Adipsin
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hbt is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Infectious diseases, Nephrology
Protocol CoA-TDS HM2200 - 2300
HM2258
74 kb

Calculate your ELISA data easily

With the ELISA calculator you can easily calculate ELISA data. Assayfit Pro helps to perform curve fitting. The calculator generates advanced reports, fit graph, fit parameters and goodness of fit are shown.

Hycultbiotech_contact us

Would you like some virtual coffee?

Contact us