The monoclonal antibody 7E9 recognizes mouse complement receptors type 1 (CR1) and 2 (CR2). CR1 and CR2 are cell surface glycoproteins that are capable of binding to activation fragments of the third and/or fourth complement components (C3 and/or C4). They play a role in the clearance of immune-complexes, phagocytosis, complement regulation, and immunoregulation. Mouse CR1 (MCR1, 190 kD) is found on the surface of B-lymphocytes, follicular dendritic cells and at lower levels on peritoneal macrophages and activated granulocytes. MRC1 has binding activity for C3b and serves as a cofactor for factor I-mediated cleavage of C3b. Mouse CR2 (MCR2, 150 kD) is a type I transmembrane glycoprotein that binds complement fragments (C3d(g), iC3b) and interferon (IFN)-alpha. MCR2 is expressed on B-lymphocytes and probably on follicular dendritic cells. On human B lymphocytes it acts as the Epstein-Barr virus (EBV) receptor. MCR2 mediates the formation of rosettes between B-lymphocytes and E-bearing Crd. MCR1 and MRC2 are very closely related. They are both products of a single gene, Cr2, formed by alternative splicing of mRNA. MCR2 corresponds to the carboxy-terminal portion of MCR1. This is in contrast with human CR1 (CD35) and CR2. The monoclonal antibody 7E9 does not inhibit rosette formation between 2PK3 cells and Crd-coated SRBC indicator cells.
Flow cytometry, Immuno fluorescence, Immuno precipitation, Western blot
For flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.