Elastase, Human, mAb 265-3K1
The monoclonal antibody 265-3K1 recognizes human leukocyte elastase. Leukocyte elastase, a major serine proteinase in man, is predominantly present in the azurophilic granules of neutrophils and monocytes. Elastase has a broad range of extracellular matrix substrates including elastin, proteoglycans, collagen and fibronectin. The action of elastase is controlled by serine proteinase inhibitors. Elastase, when released during inflammation, is rapidly bound by its two main inhibitors, alpha1-PI and alpha2-macroglobuline to form elastase-inhibitor complexes. In addition mucosa secretions may contain the locally secreted elastase inhibitors elafin/SKALP and SLPI. When secreted at sites of inflammation elastase can cause severe tissue damage. An important role has been suggested for human elastase in various inflammatory disorders, including pulmonary emphysema, sepsis, arthritis, nephritis and certain skin diseases. Elastase induces the production of IL-8 in human bronchial epithelial, a proces that occurs in part through TLR4.
FC: cells were fixed with ADG fixation buffer A for 15 min at room temperature. Cells were washed and incubated with 10 μg of monoclonal anti-HNE antibody diluted in ADG permeabilisation buffer B (Ref.1).
IF: tissues were fixed in paraformaldehyde (PFA) 3.7%, embedded in paraffin and sectioned at 6 mm. The sections were deparaffinized in toluene and hydrated in graded series of ethanol (Ref.2).
Calculate your ELISA data easily
With the ELISA calculator you can easily calculate ELISA data. Assayfit Pro helps to perform curve fitting. The calculator generates advanced reports, fit graph, fit parameters and goodness of fit are shown.
We are glad to support you!
Our technical support team of qualified scientists is more than willing to assist.