Monoclonal antibody HM2380 clone 3F12E7 recognizes human and mouse glycoprotein fibroblast growth factor 2 (FGF2), which is a member of the growth factor family involved in a variety of biological processes like differentiation, proliferation and (tumor) angiogenesis.
FGF2 exists as 5 low to high molecular weight isoforms differing in their Nterminal extensions and with distinct intracellular location and function.
The conserved 18 kDa form is most widely investigated and is predominantly cytosolic and acts through cell surface receptors like FGFR1-4. Besides it has several other binding partners that mediates its function such as heparin, integrins and PF4. The FGF2 receptors are abundant expressed on cells in the tumor environment like (activated) endothelial cells.
The protein is involved in normal processes such as wound healing, cell and organ differentiation and angiogenesis, but also in disease as a result of inflammation or in tumor growth. Emerging data postulates FGF2 pathway activation as a counteracting mechanism from inhibition from angiogenesis via VEGF.
The antibody can be used in western blotting and blocks proliferation in vitro and tumor growth in vivo.
Functional studies, Immuno assays, Western blot
IA: Antibody 3F12E7 can be used as capture and detection antibody
W: Reduced conditions were used. The expected band size is ~17 kDa
FS: Anti-FGF2 3F12E7 mAb can neutralize the FGF2-driven activity
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.