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Catalog # HM2380

FGF2, Human, mAb, clone 3F12E7


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Monoclonal antibody HM2380 clone 3F12E7 recognizes human and mouse glycoprotein fibroblast growth factor 2 (FGF2), which is a member of the growth factor family involved in a variety of biological processes like differentiation, proliferation and (tumor) angiogenesis.
FGF2 exists as 5 low to high molecular weight isoforms differing in their Nterminal extensions and with distinct intracellular location and function.
The conserved 18 kDa form is most widely investigated and is predominantly cytosolic and acts through cell surface receptors like FGFR1-4. Besides it has several other binding partners that mediates its function such as heparin, integrins and PF4. The FGF2 receptors are abundant expressed on cells in the tumor environment like (activated) endothelial cells.
The protein is involved in normal processes such as wound healing, cell and organ differentiation and angiogenesis, but also in disease as a result of inflammation or in tumor growth. Emerging data postulates FGF2 pathway activation as a counteracting mechanism from inhibition from angiogenesis via VEGF.

The antibody can be used in western blotting and blocks proliferation in vitro and tumor growth in vivo.

Functional studies, Immuno assays, Western blot

Application Notes
IA: Antibody 3F12E7 can be used as capture and detection antibody W: Reduced conditions were used. The expected band size is ~17 kDa FS: Anti-FGF2 3F12E7 mAb can neutralize the FGF2-driven activity

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
100 µg, 20 µg, 500 µg
0.2 µm filtered in PBS+0.1%BSA
Human FGF2 cDNA
Mouse IgG1
Cross reactivity
Mouse – Yes
Fibroblast Growth Factor 2
Entrez Gene ID 2257 Uniprot P09038
Tumor Immunology
Country of Manufacture
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
CoA-TDS Safety Data Sheet

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