Home \ Antibodies \ FHR-4, Human, mAb clone 4A.8
Catalog # HM2438

FHR-4, Human, mAb clone 4A.8

This monoclonal is unique, since it is not cross reactive with any of the seven proteins that belong to the Factor H (FH)-protein family.

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Clone 4A.8 recognizes human full-length Complement Factor H-related protein 4 (FHR-4). FHR-4 is a glycoprotein that is part of the complement regulatory protein family. It is synthesized and secreted primarily by the liver, as well as immune cells such as monocytes, macrophages, and dendritic cells. Unique among the FHR proteins, FHR-4 has two splice variants: FHR-4A, a longer protein with an approximate molecular weight of 86 kDa composed of 9 short consensus repeats (SCR) domains, and FHR-4B, a shorter protein of around 42 kDa with 5 SCR domains. HM2438 is an antibody that recognizes both variants, FHR-4A and FHR-4B.

Structurally, FHR-4 shares homology with Complement Factor H (CFH), a key regulator within the complement system. FHR-4 operates within the complement regulatory network, particularly affecting the alternative pathway (AP) of the complement system. It binds C3b, thereby participating in the regulation of AP activation. FHR-4 also plays a role in the opsonization of necrotic cells, facilitating the binding and recruitment of CRP.

Elevated systemic levels of FHR-4 have been strongly associated with age-related macular degeneration (AMD). However, studies have also reported inconclusive or contradictory results regarding the involvement of FHR-4 in AMD. This ambiguity highlights the need for further research to clarify FHR-4’s role in AMD and potentially other diseases. FHR-4’s unique structural properties and its diverse biological functions emphasize its significance in the complement system and its potential as a target for therapeutic interventions.

Western blot

Application Notes
W: A reduced sample treatment and SDS-Page was used. The band size for FHR-4a is ~86 and 150 kDa. For FHR-4b the band size is ~43 kDa.

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
100 µg, 20 µg
100 µg/ml 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Human Complement factor H-related protein 4
Mouse IgG1
Autoimmunity, Nephrology
Country of Manufacture
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
CoA-TDS Safety Data Sheet Folders

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