Home \ Antibodies \ H-FABP, Human, mAb 66E2
H-FABP, Human, mAb 66E2-0
Catalog # HM2016

H-FABP, Human, mAb 66E2

120,001.158,00

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The monoclonal antibody 66E2 recognizes human heart fatty acid binding protein (H-FABP) of both natural and recombinant origing. The H-FABP protein is derived from the human FABP3 gene. FABPs are small intracellular proteins (~13-14 kDa) with a high degree of tissue specificity that bind long chain fatty acids. They are abundantly present in various cell types and play an important role in the intracellular utilization of fatty acids, transport and metabolism. There are at least nine distinct types of FABP, each showing a specific pattern of tissue expression. Due to its small size, FABP leaks rapidly out of ischemically damaged necrotic cells leading to a rise in serum levels. Ischemically damaged tissues are characterized histologically by absence (or low presence) of FABP facilitating recognition of such areas. H-FABP is localized in the heart, skeletal and smooth muscle, mammary epithelial cells, aorta, distal tubules of the kidney, lung, brain, placenta, and ovary. The monoclonal antibody 66E2 stains heart muscle cells and striated skeletal muscle cells in immunohistology. It can be used to detect ischemia areas of human heart. It is also useful as marker for brain damage. Furthermore, this antibody is useful for the purification of H-FABP.

Application
Frozen sections, Immuno assays, Immuno precipitation, Western blot

Application Notes
IP: Biotinylated 66E2 was immobilized on streptavidin beads and added to serum to immunoprecipitate H-FABP (Ref.5).
W: Reduced sample treatment. The band size is ~15 kDa (Ref.4). 1μg/ml was used (Ref.6).
F: Permeabilization was done in cold acetone with 0.5% hydrogen peroxidase for 10 min, after drying and washing, antibodies were incubated for 30 minutes.

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Positive Control
Heart cells or recombinant H-FABP

Negative Control
Tonsil tissue

Product type
Monoclonal antibodies
Quantity
20 µg, 100 µg, 500 µg
Formulation
Quantities >500 µg will be send without preservative and carrier free. Quantities <500 µg will be send with preservative and carrier. exact formulation can be found on the datasheet.
Immunogen
Purified human H-FABP
Isotype
Mouse IgG1
Species
Human
Cross reactivity
Mouse – Yes, Rat – Yes, Swine – Yes
Alias
Heart-type fatty acid-binding protein, Fatty acid-binding protein 3, FABP3, Muscle fatty acid-binding protein, Mammary-derived growth inhibitor<br> - Gene name: FABP3, FABP11, MDGI
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product.
Disease
Cardiology and metabolism, Neurological disorders

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