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Catalog # HK508


Increased window of detection, results in hours instead of days!

The EcHECk anti-EHEC O157:H7 IgM assay is a serological assay for standardized detection of antibodies against Enterohemorrhagic Escherichia coli (EHEC) O157:H7 LPS.

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Increased window of detection, results in hours instead of days!

Developed in cooperation with; Radboud University Medical Center (NL) and the University Clinic of Münster (DE) 

The EcHECk ELISA enables reliable detection of IgM antibodies against EHEC bacteria in serum up to two months after onset of infection. Detection of EHEC is currently performed by PCR or culturing and these methods are not able to identify EHEC after one week of infection.

Using this EcHECk ELISA kit to determine a possible EHEC infection will offer you a reliable diagnosis for a longer time frame and start the correct treatment.  

EHEC (or Shiga-toxin producing Escherichia coli; STEC), serotype O157:H7, is recognized worldwide as a dangerous bacterial pathogen responsible for foodborne infections in humans. EHEC contamination occurs through:

– The ingestion of contaminated food or water

– Person-to-person transmission

– Contact with ruminants or their contaminated environment.

The main symptoms are abdominal cramps and diarrhea, which is bloody in about 60% of patients and occurs after a median incubation period of three days. About 5–10% of people diagnosed with STEC O157 infection develop a life-threatening complication known as hemolytic uremic syndrome (STEC-HUS).This disease is defined by mechanical hemolytic anemia, thrombocytopenia, and acute renal injury and may lead to renal failure.

EHEC infections are detected by an examination of fecal material, mostly through simultaneous testing of stool culture, shiga toxins immunoassays, and/or polymerase chain reaction PCR assays for the detection of shiga toxin (Stx) genes.

However, the presence of EHEC in the intestines declines rapidly during the first 7 days of illness, whereas the average time between the first day of diarrhea and the development of HUS is approximately 5-13 days.

As a result, examination of stool samples and/or PCR are only able to detect EHEC O157 infections for a short period of time after disease onset. In contrast, serum IgM antibodies to lipopolysaccharide (LPS) of EHEC O157:H7 can be detected from 5 days up to 2 months after onset of the symtoms.

The anti-EHEC O157:H7 IgM assay (cat# HK508) is a robust serological assay for standardized detection of these IgM antibodies against EHEC O157:H7 LPS and can be used as an additional method improving the detection of EHEC infections.

The key advantages:

– Reliable fast and extended detection of EHEC infection

– Prevention of medical escalation

– Save time and costs

The EcHECk ELISA kit is to be used for the in vitro qualitative determination of anti-EHEC O157:H7 IgM in serum and plasma samples.

The EcHECk ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay.

Samples and standards are incubated in microtiter wells coated with LPS recognizing IgM EHEC O157:H7. Biotinylated tracer antibody will bind to the captured Human-anti-EHEC O157:H7 IgM. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid.

The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the EcHECk standards (log).

Product type
1 x 96 det.
Standard range
1.6 to 100 AU/ml
Detection level
1.6 AU/ml
Working volume
100 µl/well
EHEC; Enterohemorrhagic Escherichia coli; serotype O157:H7; Shiga-toxin producing Escherichia coli; STEC
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Infectious diseases
Safety Data Sheet Assay Manual
468 kb
Product information English

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