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Catalog # HM1089

M-CSF-responsive cells, Mouse, mAb ER-MP58

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The monoclonal antibody ER-MP58 recognizes an antigen present on all mouse macrophage colonystimulating factor (M-CSF) responsive cells in the bone marrow, including the earliest colony-forming myeloid progenitors, as well as by the majority of other myeloid precursors. The antigen is detected on a broad development range of macrophage precursor cells to the monocytic level, but also on granulocytes. Expression is rapidly lost upon maturation beyond the monocytic stage. The antigen disappears in the course of macrophage differentiation. Furthermore the antigen is clearly different from commonly used myeloid markers as Mac-1, F4/80, and Gr-1.
The monoclonal antibody ER-MP58 is very useful for the identification of mouse myeloid hematopoietic islands in various organs, and for embryonic studies. Cells committed to the myeloid lineage can be separated from progenitor cells with other differentiation capacities by means of multiparameter cell sorting using monoclonal antibody ER-MP58 in combination with monoclonal antibody ER-MP12 (HM1084) and ER-MP-20 (HM1082).

Application
Flow cytometry, Frozen sections

Use
For immunohistology and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Isotype
Rat IgG2a
Species
Mouse
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product.
Disease
Cardiology and metabolism, Nephrology
Protocol
Protocol Flow Cytometry
58 kb
Protocol IHC-F
36 kb
CoA-TDS HM1067-HM2099
HM1089-100UG
80 kb
HM1089-20UG
101 kb
Safety Data Sheet
SDS - HM1089-100UG
52 kb
SDS - HM1089-20UG
52 kb

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