Home \ Antibodies \ MHC class I, Mouse, mAb ER-MP42
Placeholder
Catalog # HM1091

MHC class I, Mouse, mAb ER-MP42

Please choose the quantity of items you wish to add to your cart…
Find distributor

The monoclonal antibody ER-MP42 specifically reacts with major histocompatibility complex (MHC) class I antigens of the mouse and, therefore, it is a valuable tool for studying cytotoxic T-cell interactions with class I positive antigen presenting cells. The primary immunological function of MHC molecules is to bind and present antigenic peptides on the surfaces of cells for recognition by the antigen-specific T cell receptors (TCRs) of lymphocytes. MHC class I molecules are expressed on the surfaces of most cells and are recognized by CD8-positive cytotoxic T-cells, an essential step for initiating the elimination of virally infected cells by T-cell-mediated lysis. MHC class I molecules are heterodimers composed of an alpha (44kD) and a beta (beta microglobulin, 11 kD) subunit. The first two structural domains of the alpha subunit associate to form the peptide-binding pocket. The monoclonal antibody ER-MP42 reacts with MHC class I, an antigen that is expressed by all somatic cells at varying levels. Lymphocytes are highly positive, whereas fibroblasts and neurons show only a low level of antigen expression. ER-MP42 recognizes murine MHC class I molecules on the surface of cells of the following haplotypes: H-2Fv, H-2Dd, H-2k,q,s. Weaker reactivity is found in mice of the following haplotypes: H-2p,r,w7,w22. MHC class I molecules of other haplotypes are not recognized by ER-MP42. There is no cross-reactivity with human MHC class I molecules.

Application
Flow cytometry, Frozen sections

Use
For immunohistology and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg
Formulation
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Isotype
Rat IgG2a
Species
Mouse
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease
Infectious diseases
Protocol CoA-TDS HM1067-HM2099 Safety Data Sheet

Calculate your ELISA data easily

With the ELISA calculator you can easily calculate ELISA data. Assayfit Pro helps to perform curve fitting. The calculator generates advanced reports, fit graph, fit parameters and goodness of fit are shown.

Latest Hycult Biotech news

  • New Human Complement Pathway Assays
    We are very proud of our newly developed human classical and alternative complement pathway assays. They are produced in response to a growing demand for quantitative investigation of complement inhibitors or regulators at lower sample dilutions. This development aims to address the issue of false negative results, enabling more accurate and reliable analysis of complement… Read more: New Human Complement Pathway Assays
  • Navigating the pitfalls in complement analysis
    Our colleague Erik Toonen shared his experience on how to analyze complement at the Complement-based Drug Development Summit in Boston in September 11-13th, 2023. He showed valuable insights on analyzing complements. For accurate complement analysis, it is important that not only the correct technique is used but also that pre-analytical sample handling is performed in… Read more: Navigating the pitfalls in complement analysis
  • NEW Human C3d ELISA
    We are happy to introduce our new ELISA (cat.# HK3017) designed to detect human C3d. What sets this assay apart is its utilization of a distinctive neo-epitope capture antibody that selectively targets a specific C3d region of the alpha chain, which is not available in other C3 variants. This way it specifically distinguishes C3d from native C3… Read more: NEW Human C3d ELISA
Contact us