Post-translational modification regulated by conjugation of a small ubiquitin-like modifier (SUMO) is involved in various cellular processes such as transcriptional regulation, apoptosis and nuclear transport. Recent proteomic analyses in mammalian cells revealed that a number of SUMO substrates and specific modifications by SUMO-1 are involved in essential processes, including chromatin organization, transcription, RNA metabolism, immunomodulation and development of the innate immune system.
Immuno assays, Paraffin sections, Western blot
Higher expression of SUMO-1 was detected in normal adrenal gland, esophagus, pancreas, liver, stomach, kidney, and uterus. the function of SUMO-1 may vary depending on the tissue types.
IHC-P: Tissue sections were deparaffinized in xylene, rehydrated, and treated with fresh 0.3% hydrogen peroxide in methanol for 15 min.
W: A non-reduced sample treatment was used.
For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.