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Catalog # HM2218

TNF-alpha, Human, mAb 5N

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The monoclonal antibody 5N reacts with Tumor Necrosis Factor alpha (TNF-alpha). Tumor necrosis factor-alpha (TNF-alpha), a homotrimeric 17 KD protein, is a potent mediator of inflammatory and metabolic functions. TNF-alpha was originally detected as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Further TNF-alpha has been implied as central mediator in shock induced by gram negative micro-organisms. The cytokine TNF-alpha is found to be a central mediator in many inflammatory and immunological processes: it can be induced by various products of micro-organisms and by various cytokines but it also induces on its turn the production of many cytokines. Signal transduction occurs via two types of TNF-receptors, the TNF-receptors I and II. The receptors differ strongly in their intra-cellular signaling pathways. The TNF-alpha trimer interacts with either of the two types of TNF-R leading to receptor cross-linking. The monoclonal antibody 5N cross-reacts and cross-neutralizes all monkey species TNF-alpha, except pig-tail macaque. With affinity about two orders lower it recognizes bovine and canine TNF-alpha.

Application
Functional studies, Immuno assays

Use
For neutralization of biological activity in vitro dilutions have to be made according to the amounts of TNF-alpha to be inactivated. The affinity of the monoclonal antibody 5N is 5.7 x 10-10M. The homologous ELISA is useful for the detection of oligomeric human TNF-alpha. It does not recognize monomeric TNF-alpha.

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg, 500 µg
Formulation
Quantities >500 µg will be send without preservative and carrier free. Quantities <500 µg will be send with preservative and carrier. exact formulation can be found on the datasheet.
Isotype
Mouse IgG2a
Species
Human
Cross reactivity
Bovine – Yes, Canine – Yes, Monkey – Yes
Alias
TNF, TNF-SF2, DIF, Cachectin
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Autoimmunity, Gastroenterology
Protocol CoA-TDS HM2200 - 2300
HM2218
54 kb
Safety Data Sheet

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