New

CLM-5, Mouse, clone TX69

Quantity:
20 µg
Catalog #:
HM1147-20UG

Original supplier of innate immunity products since 1994.

Our commitment to quality: Read more!

Contact our support team for more product information.

Can't find your application for a product? Request a sample!

HM1147 (clone TX69) is a monoclonal antibody against mouse CLM-5.This molecule, also known as MAIR-IV, LMIR4 or CD300ld1, is a member of the myeloid-associated immunoglobulin-like receptor family (MAIR, CD300). The CD300 family of receptors is an evolutionary conserved receptor family that belongs to the Ig superfamily and is expressed predominantly by the myeloid lineage. In mice, nine members have been reported with different nomenclature systems, such as myeloid-associated Ig-like receptor (MAIR), leukocyte mono-Ig-like receptor (LMIR), DC-derived Ig-like receptor (DIgR), and CMRF-35-like molecule (CLM). CD300 molecules act as either activating or inhibitory receptors through interactions with adaptor molecules, such as PAD12, DAP10, and FcRγ. CLM-5 is preferentially expressed on Gr-1+/Mac-1+ neutrophils in the peripheral blood, bone marrow, peritoneal cavity, and spleen. It is also found on Mac-1+/Gr-1+ macrophages and CD11c+ dendritic cells. Its expression is regulated by TLR ligands LPS and flagellin. In association with the immunoreceptor tyrosine based activating motif (ITAM)-bearing adaptor FcRγ, CLM-5 mediates activation signals for neutrophils thereby playing an important role for innate immunity such as the induction of proinflammatory cytokines TNF-a and IL-6.
Specifications
Product type Monoclonal antibodies
Quantity 20 µg
Formulation 0.2 µm filtered in PBS+0.1%BSA+0.02%NaN3
Application Flow cytometry, Western blot
Immunogen Mouse T cell leukemia cell line BW5147 transfected with Flag-tagged MAIR-IV (Ref.1)
Isotype Rat IgG2a
Species Mouse
Alias CMRF35-like molecule 5, MAIR-IV, CD300 antigen-like family member D, Leukocyte mono-Ig-like receptor 4, Myeloid-associated immunoglobulin-like receptor 4, CD300ld1.
References 1. Nakano, T et al; Activation of neutrophils by a novel triggering immunoglobulin-like receptor MAIR-IV. Mol Immunol 2008; 45: 289
Storage and stability Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Applications
Application: Flow cytometry, Western blot
Application notes: • FC: Extracellulair staining of CLM-5 with TX69.
• W: Western blot was performed under reduced conditions. The expected band size is 35 kDa.
References
References: 1. Nakano, T et al; Activation of neutrophils by a novel triggering immunoglobulin-like receptor MAIR-IV. Mol Immunol 2008; 45: 289
CoA-TDS HM1067-HM2099
pdf
(Size: 0.24 MB)