Allergin-1, Mouse, clone TX83

Monoclonal antibody clone Tx83 recognizes mouse allergy inhibitory receptor-1 (Allergin-1; also known as mast cell antigen AG-32)
Quantity:
100 µg
Catalog #:
HM1143-100UG

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Monoclonal antibody clone Tx83 recognizes mouse allergy inhibitory receptor-1 (Allergin-1; also known as mast cell antigen AG-32). It is an immune regulatory molecule predominantly expressed on mast cells, but also found on other myeloid cells like macrophages, dendritic cells and neutrophils. The receptor consist of an immunoreceptor tyrosine-based inhibitory motif (ITIM) like domain. It’s main function is to suppress IgE (FcεRI-) mediated degradation and TLR2- mediated signaling in mast cells. Thereby inhibiting production of cytokines and other inflammatory mediators. Allergin-1 is tyrosine phosphorylated and recruits SH2 phosphatases , such as SHP-1, SHP-2 or SHIP. Co-ligation with FcεRI decreases mast cell degranulation. Currently three isoforms of human allergin-1 are identified: allergin-1L, Allergin-1S1 & allergin-1S2. Mast cells play a pivotal role in allergic reactions in barrier tissues, like skin and intestinal mucosa. Allergin-1 is suggested to play a role in skin diseases like dermatitis, the autoimmune diseases such as SLE and airway hyperresponsiveness. In macrophages it is suggested to play a role by suppressing autoantibody production via the promotion of apoptotic cell clearance.
Specifications
Product type Monoclonal antibodies
Quantity 100 µg
Formulation 0.2 µm filtered in PBS+0.1%BSA+0.02%NaN3
Application Flow cytometry
Immunogen TX83 was generated by immunizing Allergin-1-deficient mice with BW5147 transfectants expressing Flag-tagged Allergin-1 (Ref.1).
Isotype Mouse IgG1
Species Mouse
Alias allergy inhibitory receptor-1 , mast cell antigen AG-32
References 1. Hitomi, K et al; A novel immunoglobulin-like receptor, Allergin-1, inhibits immunoglobulin E-mediated immediate hypersensitivity reactions. Nat. Immunol. 11:601.
2. Tsurusaki, S et al; Allergin-1 inhibits TLR2-mediated mast cell activation and suppresses dermatitis. Int Immunol. 2016, 28:605.
3. Hitomi, K et al; Allergin-1 on mast cells suppresses house dust mite-induced airway hyperresponsiveness in mice. Int Immunol 2018.
4. Iizuka, A et al; Allergy inhibitory receptor‐1 inhibits autoantibody productionvia upregulation of apoptotic debris clearance by macrophages. Rheum Dis 2018, 21:2071.
Storage and stability Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Applications
Application: Flow cytometry
References
References: 1. Hitomi, K et al; A novel immunoglobulin-like receptor, Allergin-1, inhibits immunoglobulin E-mediated immediate hypersensitivity reactions. Nat. Immunol. 11:601.
2. Tsurusaki, S et al; Allergin-1 inhibits TLR2-mediated mast cell activation and suppresses dermatitis. Int Immunol. 2016, 28:605.
3. Hitomi, K et al; Allergin-1 on mast cells suppresses house dust mite-induced airway hyperresponsiveness in mice. Int Immunol 2018.
4. Iizuka, A et al; Allergy inhibitory receptor‐1 inhibits autoantibody productionvia upregulation of apoptotic debris clearance by macrophages. Rheum Dis 2018, 21:2071.
CoA-TDS HM1067-HM2099
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