C3 itself is a pivotal 190 kD protein central to the complement system, integral to the classical, alternative, and lectin pathways of complement activation. Notably, its synthesis is tissue-specific and responds dynamically to various stimulatory agents. Being the most abundant complement system protein, with serum levels around 1.3 mg/ml, C3’s activation is a key event in immune response.
Activation of C3, a critical step identified by the activated C3 antibody, involves its cleavage into C3a and C3b. C3a plays a vital role in mediating local inflammatory processes, with notable anaphylatoxic properties. It induces smooth muscle contraction, increases vascular permeability, and triggers histamine release from mast cells and basophils. Conversely, C3b attaches to immune complexes and undergoes further cleavage into iC3b, C3c, C3dg, and C3f. Collectively, these fragments are known as activated C3 (act. C3).
The most significant function of these C3 fragments is their interaction with other immune cells. The neo-epitopes formed during activation, which the activated C3 antibody detects, are vital for understanding the complement cascade’s impact on the immune system. Monoclonal antibodies like I3/15, which target these neo-epitopes, have become essential tools for directly quantifying activation at different steps of the complement cascade.
Studying the activated C3 antibody’s role in C3 activation is key for immune response insights and therapies.
Not sure which C3 antibody to use?
With numerous options available, it is essential to select the right C3 antibody to ensure the success of your research. We designed a guide to assist you in making an informed decision:
Go to our C3 researcher’s guide and choose the right antibody
Immuno assays, Western blot
For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.