Home \ Antibodies \ Activated C3, Human, mAb I3/15
Catalog # HM2257

Activated C3, Human, mAb I3/15

The activated C3 antibody, specifically the monoclonal antibody I3/15, plays a crucial role in recognizing the activated complement protein C3. This recognition includes a unique neo-epitope present on C3b, iC3b, and C3dg, distinct from native C3. The I3/15 antibody’s ability to identify native C3 after activation unfolding or post-SDS treatment is significant in understanding complement activation.


Read more


Please choose the quantity of items you wish to add to your cart…
Find distributor

C3 itself is a pivotal 190 kD protein central to the complement system, integral to the classical, alternative, and lectin pathways of complement activation. Notably, its synthesis is tissue-specific and responds dynamically to various stimulatory agents. Being the most abundant complement system protein, with serum levels around 1.3 mg/ml, C3’s activation is a key event in immune response.

Activation of C3, a critical step identified by the activated C3 antibody, involves its cleavage into C3a and C3b. C3a plays a vital role in mediating local inflammatory processes, with notable anaphylatoxic properties. It induces smooth muscle contraction, increases vascular permeability, and triggers histamine release from mast cells and basophils. Conversely, C3b attaches to immune complexes and undergoes further cleavage into iC3b, C3c, C3dg, and C3f. Collectively, these fragments are known as activated C3 (act. C3).

The most significant function of these C3 fragments is their interaction with other immune cells. The neo-epitopes formed during activation, which the activated C3 antibody detects, are vital for understanding the complement cascade’s impact on the immune system. Monoclonal antibodies like I3/15, which target these neo-epitopes, have become essential tools for directly quantifying activation at different steps of the complement cascade.

Studying the activated C3 antibody’s role in C3 activation is key for immune response insights and therapies.

Not sure which C3 antibody to use?
With numerous options available, it is essential to select the right C3 antibody to ensure the success of your research. We designed a guide to assist you in making an informed decision:

Go to our C3 researcher’s guide and choose the right antibody

Immuno assays, Western blot

For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
100 µg, 20 µg
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Mouse IgG1
C3, PZP-like alpha-2-macroglobulin domain-containing protein 1
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Infectious diseases, Nephrology

You may also like…

You may be interested in…

Calculate your ELISA data easily

With the ELISA calculator you can easily calculate ELISA data. Assayfit Pro helps to perform curve fitting. The calculator generates advanced reports, fit graph, fit parameters and goodness of fit are shown.

Contact us