Home \ Antibodies \ C3/C3a, Human, mAb 2898
Catalog # HM2075

C3/C3a, Human, mAb 2898

The monoclonal antibody 2898 is uniquely designed to recognize the C-terminus of human complement protein C3a. This antibody’s capability extends to identifying intact C3, C3a, and its derivative, C3a-desArg, highlighting its importance in the study of C3/C3a in the immune system.

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Abundant and Crucial in the Complement System C3, the most abundant protein in the complement system, plays a central role in innate immunity with serum levels approximately 1.3 mg/ml. As a critical component of the complement system, which includes three pathways, C3 and its fragment C3a are integral to both the classical, alternative, and lectin pathways of complement activation.

The Activation and Function of C3/C3a

The activation of C3 leads to the formation of various products, including C3a, which bear neo-epitopes absent in their native form. Monoclonal antibodies like the 2898 are instrumental in directly quantifying these activation stages. Tissue-specific synthesis of C3, responsive to numerous stimulants, further underscores the dynamic nature of C3/C3a in immune responses. Moreover, a deficiency in C3 can predispose individuals to frequent bacterial infections, emphasizing the protective role of C3/C3a in the immune system. This is particularly evident in conditions like ulcerative colitis and chronic inflammatory bowel disease, where abnormal C3 deposition is observed.

Proteolytic Cleavage and the Stability of C3a

Enzymatic proteolysis of C3 releases the anaphylatoxin C3a and C3b. The C3a fragment, a protein of 74 amino acids, is inherently short-lived, quickly transforming into the more stable form, C3a-desArg. This transformation makes C3a-desArg a reliable marker for complement activation levels in test samples, showcasing the significance of C3/C3a in immunological assays.

C3a’s Role in Inflammatory Responses

C3a is a potent mediator in local inflammatory processes. It induces smooth muscle contraction, increases vascular permeability, and triggers histamine release from mast cells and basophils. C3a mediates inflammation, impacting conditions like sepsis, ischemic heart disease, and autoimmune diseases.


The comprehensive understanding of C3/C3a, from its detection by monoclonal antibodies to its role in immune responses and diseases, is crucial. The study of C3/C3a not only illuminates key aspects of the immune system but also aids in the development of targeted therapeutic interventions.

Not sure which C3 antibody to use?
With numerous options available, it is essential to select the right C3 antibody to ensure the success of your research. We designed a guide to assist you in making an informed decision:

Go to our C3 researcher’s guide and choose the right antibody

Immuno assays, Western blot

Application Notes
W: A reduced sample treatment and SDS-PAGE was used. The band size is ~95 and 8.2 kDa for C3 and C3a respectively (Ref.1).

For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Positive Control
Human plasma

Product type
Monoclonal antibodies
100 µg, 20 µg, 500 µg
Quantities >500 µg will be send without preservative and carrier free. Quantities <500 µg will be send with preservative and carrier. exact formulation can be found on the datasheet.
Peptide CARASHLGLA (last nine amino acids of C3a-desArg)
Mouse IgG1
Complement protein C3
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Infectious diseases, Nephrology

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