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Catalog # HM2073

C3/C3a, Human, mAb 474

One of the key agents interacting with complement protein C3/C3a is monoclonal Antibody 474. This antibody is notable for its specific reactivity with an epitope on C3a, demonstrating its effectiveness both with intact C3 and C3a fragments. This interaction is crucial for understanding the dynamics of the complement system.

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Understanding Complement Protein C3/C3a and its Role in Innate Immunity

The complement system, a crucial component of innate immunity, includes a pivotal element known as complement protein C3/C3a. This protein plays a central role in the classical, alternative, and lectin pathways of complement activation, underlining its importance in the immune response.

The Synthesis and Function of Complement Protein C3/C3a

Complement protein C3/C3a is synthesized in a tissue-specific manner, influenced by various stimulatory agents. A significant aspect of C3/C3a’s function involves its cleavage during complement activation. This cleavage releases C3a, an anaphylatoxic peptide consisting of 74 amino acids, approximately 10 kDa in size. C3a, known for its transient nature in serum, is quickly converted into a more stable form, C3a-desArg (also referred to as acylation stimulating protein, ASP).

C3a’s Mechanism of Action

C3a operates through a specific receptor, C3aR, belonging to the G-protein coupled receptor family. This receptor’s expression across various cell types, including myeloid and non-myeloid cells, underscores its broad impact. Notably, C3aR’s presence on hematopoietic stem/progenitor cells enhances their trafficking to the bone marrow. Additionally, C3aR’s engagement with dendritic cells (DCs) and T cells has been shown to augment their functions.

C3a’s Role in Inflammatory Processes

Complement protein C3/C3a, particularly C3a, is a key mediator in local inflammatory processes. It induces smooth muscle contraction, increases vascular permeability, and prompts histamine release from mast cells and basophilic leukocytes. The involvement of C3a in inflammatory responses is evident in conditions such as gram-negative bacterial sepsis, ischemic heart disease, and various autoimmune diseases.

Not sure which C3 antibody to use?
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Immuno assays, Western blot

Application Notes
W: diabetic serum proteins were separated on PAGE-gel and transferred to nitrocellulose

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Positive Control
Human serum

Negative Control
C3-deficient serum

Product type
Monoclonal antibodies
100 µg, 20 µg, 500 µg
Quantities >500 µg will be send without preservative and carrier free. Quantities <500 µg will be send with preservative and carrier. exact formulation can be found on the datasheet.
Mouse IgG1
Complement component 3
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Infectious diseases, Nephrology

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