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Catalog # HM2072

C3/C3b, Human, mAb 755

The monoclonal antibody 755 plays a pivotal role in understanding C3/C3b. It recognizes an epitope within the C-terminal 360 amino acids of the alpha chain of C3. This specific recognition allows it to identify both C3b and full C3, providing valuable insights into the functionality of C3/C3b.

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Exploring the Role of C3/C3b in Innate Immunity and Complement Activation

The complement system, a cornerstone of innate immunity, involves crucial components like C3/C3b. This key element is central to the classical, alternative, and lectin pathways of complement activation, highlighting its significance in immune defense mechanisms.

Synthesis and Abundance

The synthesis of C3, is tissue-specific and influenced by various stimulatory agents. As the most abundant protein in the complement system, C3 has significant serum protein levels, about 1.3 mg/ml. Understanding the synthesis and regulation of C3 helps in comprehending the dynamics of C3/C3b.

Disease and Immune Response

A deficiency in C3/C3b can lead to increased susceptibility to bacterial infections, underlining its protective role. In contrast, the deposition of C3 fragments, including C3/C3b, is observed in diseases like ulcerative colitis and chronic inflammatory bowel disease, indicating its role in immunopathology.

The Mechanism of C3/C3b Formation and Function

Upon activation of the complement system, enzymes cleave C3 into C3b (part of C3/C3b) and C3a. C3b binds to immune complexes and undergoes further cleavage into various fragments. Within the alternative pathway, C3b is essential for amplifying the immune response, highlighting the critical role in this process.

Not sure which C3 antibody to use?
With numerous options available, it is essential to select the right C3 antibody to ensure the success of your research. We designed a guide to assist you in making an informed decision:

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Immuno assays, Western blot

Application Notes
W: A non-reduced or reduced sample treatment and SDS-PAGE was used. The band size (s) are ~190 and ~100 kDa under non-reducing and reducing conditions respectively. (Ref.1)

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Positive Control
Human serum

Negative Control
C3-deficient serum

Product type
Monoclonal antibodies
100 µg, 20 µg
0.2 ml (100 μg/ml) 0.2 μm filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Native C3
Mouse IgG1
Complement component 3
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Infectious diseases, Nephrology

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