C3a/C3a des Arg, Human, mAb 2991

Exploring the Role of C3a/C3a des Arg in Innate Immunity

The complement system is a crucial component of the humoral response in innate immunity, and it operates through three distinct pathways. At the heart of these pathways is the third complement component, C3, which is integral to the classical, alternative, and lectin pathways of complement activation. The activation products of the complement cascade, including C3a and C3a des Arg, are key to understanding this system’s function.

C3a/C3a des Arg: Understanding Its Significance

Activation products like C3a and C3a des Arg contain unique neo-epitopes, which are absent in their native forms. These epitopes are essential for direct quantification of activation at various stages in the complement cascade, often detected through monoclonal antibodies. C3, the most abundant protein in the complement system, has serum levels of about 1.3 mg/ml. Its synthesis is tissue-specific, influenced by various stimulatory agents.

The Clinical Importance of C3a and C3a des Arg

Inherited C3 deficiency can lead to frequent bacterial infections. In conditions like ulcerative colitis, C3 deposition in diseased mucosa has been observed. The cleavage of C3 by specific enzymes releases C3a anaphylatoxin and C3b. C3a, a short-lived protein of 74 amino acids, quickly transforms in the serum into the more stable form, C3a des Arg, also known as acylation stimulating protein (ASP). Measuring C3a des Arg provides reliable insights into complement activation levels in test samples.

C3a and C3a des Arg in Inflammatory Responses

C3a is a potent mediator in local inflammatory processes. It can induce smooth muscle contraction, increase vascular permeability, and trigger histamine release from mast cells and basophilic leukocytes. Its involvement is significant in conditions like gram-negative bacterial sepsis, trauma, ischemic heart disease, post-dialysis syndrome, and various autoimmune diseases. Normal plasma values of C3a/C3a des Arg in control individuals typically range between 48-150 ng/ml.

Not sure which C3 antibody to use?

With numerous options available, it is essential to select the right C3 antibody to ensure the success of your research. We designed a guide to assist you in making an informed decision:

Go to our C3 researcher’s guide and choose the right antibody