Home \ Antibodies \ C3b, iC3b, C3c, Human, clone C3-42.3
Catalog # HM2394

C3b, iC3b, C3c, Human, clone C3-42.3

The antibody clone C3-42.3 recognize complement C3, C3b, iC3b and C3c. The recognition domain is determined to MG4/5. The antibody blocks C3 activation by AP C3-convertase.


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The Integral Role of C3b, iC3b, and C3c in the Complement System and Immune Response

The complement system is a pivotal component in both innate and adaptive immune responses, capable of producing inflammatory and protective reactions to pathogenic challenges. This article highlights the significant roles of C3b, iC3b, and C3c in this vital system.

Complex Activation Pathways Leading to C3b, iC3b, and C3c Formation

Comprising a complex family of proteins and receptors, the complement system operates in circulation, tissues, and other body fluids. It activates through three pathways: the classical pathway, initiated by immune complexes; the lectin pathway, triggered by surface-bound lectins; and the alternative pathway (AP), activated by unprotected surfaces. Each pathway generates a C3 convertase, a serine protease that cleaves the central protein C3, leading to the creation of C3b.

C3 Convertases: Driving the Complement Cascade

C3 and C5 convertases are enzymatic complexes critical for initiating and amplifying complement activity, ultimately generating the cytolytic membrane attack complex (MAC). The synthesis of C3, which leads to the formation of C3b, iC3b, and C3c, is tissue-specific and responsive to various stimulatory agents. Following cleavage by C3 convertase, C3 yields anaphylotoxin C3a and the crucial fragment C3b.

The Abundance and Functionality of C3 and Its Derivatives

C3, with a molecular weight of approximately 185 kDa, is the most abundant protein in the complement system. Its primary production occurs in the liver, but it’s also generated by macrophages, neutrophils, and other cells. Due to its high levels and low biological reactivity, C3 can respond swiftly and effectively to pathogens. However, defects in C3 can lead to recurrent infections or autoimmune diseases. C3 deficiency, though rare, results in significant health challenges, including recurrent infections and impaired immune cell development. Polymorphisms in C3 have been linked to conditions like age-related macular degeneration (AMD) and atypical hemolytic uremic syndrome (aHUS).

The Significance of C3b, iC3b, and C3c in Disease and Immune Regulation

Besides pathogen clearance, C3, particularly through its fragments C3b, iC3b, and C3c, aids in the removal of circulating immune complexes, enhancing macrophages’ phagocytic capacity. Malfunctions in this system can lead to autoimmune diseases and tissue damage. Thus, understanding the roles and interactions of C3b, iC3b, and C3c is crucial in diagnosing and managing various immune-related conditions.

Not sure which C3 antibody to use?

With numerous options available, it is essential to select the right C3 antibody to ensure the success of your research. We designed a guide to assist you in making an informed decision:

Go to our C3 researcher’s guide and choose the right antibody

Functional studies, Immuno assays, Western blot

Application Notes
• IA: antibody C3-42.3 was used in a direct ELISA.
• FS: the antibody blocks C3 activation by alternative pathway C3-convertase (Ref.1)
• W: the antibody is used under reduced conditions. Expected band size is 71 kDa.

Product type
Monoclonal antibodies
100 µg, 20 µg
0.2 µm filtered in PBS+0.1%BSA
Mixture of the human activated C3 fragments, C3b, iC3b, and C3dg, emulsified in complete Freund’s adjuvant. Subsequently, mice were boosted three times at 2-week intervals with the same amount of
Mouse IgG2b
Complement component C3b, iC3b, C3c
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
CoA-TDS Safety Data Sheet

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