Home \ Antibodies \ C4, Mouse, mAb 16D2
Frozen section of mouse pulmonary artery of control (left) and mouse with hypoxia-induced pulmonary hypertension. Staining of C4 (red) with HM1046 in a 1:300 dilution. Green is autofluorescence of elastic lamellae defining vascular media, Blue is cell nuc
Catalog # HM1046

C4, Mouse, mAb 16D2

120,00462,00

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The monoclonal antibody 16D2 recognizes mouse complement factor C4, formerly known as Gg protein, which consists of an alpha-, beta-, and gamma-chain. The classical pathway of complement and the Mannose binding lectin activation pathway converge at C4. C1s, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a and C4b. Subsequently, C4b can be cleaved to C4c and C4d by other serum enzymes. The monoclonal antibody 16D2 reacts with intact C4, C4b and C4d.
C4 is an acute phase protein that is produced by hepatocytes, monocytes and intestinal epithelial cells and can be used- in experimental animals as a marker for activation of the classical complement pathway. Recent studies have demonstrated an association between graft rejection and C4d deposition in a mouse model for cardiac transplantation.

Application
Immuno assays, Immuno fluorescence, Immuno precipitation, Western blot

Application Notes
W: A non-reduced sample treatment and SDS-Page was used. The band size is 200 kDa (Ref.4).
IA: Plates were coated overnight with rat anti-murine C4 mAb 16D2 in carbonate buffer and blocked with 5% dry milk in PBS and 0.01% Tween 20 (Ref.2).
IF: Spleens were snap frozen and stored at -80°C until cryosections were cut. Sections (5 µm) were fixed for 4 min in ice-cold acetone. Sections were blocked with 2% BSA, 2% FCS, and PBS. Biotinylated 16D2 was visualized using avidin-PE (BD PharMingen, San Diego, CA) (Ref.2).

Use
For Immuno Assays, Immuno Fluorescence, Immuno Precipitation and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Positive Control
T-cell regions of mouse spleen

Product type
Monoclonal antibodies
Quantity
20 µg, 100 µg
Formulation
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Immunogen
Thymocytes decorated with Thy-1 antibody and complement components
Isotype
Rat IgG2a
Species
Mouse
Alias
Complement component 4
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Infectious diseases, Nephrology

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