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Catalog # HM2103

CD46, Human, mAb M177


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The monoclonal antibody M177 recognizes CD46, also designated membrane cofactor protein (MCP). CD46 is a 45-70 kDa protein with genetic and tissue-specific heterogeneity.- It is expressed on every cell and tissue, with the exception of erythrocytes. CD46 serves to inhibit complement activation on host tissue. It performs this function by serving as a cofactor which binds to C3b and C4b. This binding is permitted by factor I, a serine protease of plasma, to degrade C3b and C4b and serves to protect the host cell against autologous attack. It also serves as a receptor for measles virus.
Four isoforms of CD46 predominate and arise by alternative splicing of a single CD46 gene. CD46 cDNA encodes a signal sequence followed by four complement control protein domains (also called short consensus repeats (SCR)). The monoclonal antibody M177 reacts with the SCR2 domain.

Flow cytometry, Frozen sections, Functional studies, Immuno assays, Immuno precipitation, Western blot

Application Notes
FC: Antibody M177 stains the extracellular domain of CD46.
IP: 107 cells were lysed and immunoprecipitated with 25 µg M177 antibody and 25 µg protein G-sepharose
W:A non-reduced sample treatment and SDS-page was used. The band size is 45-70 kDa (Ref 1 and 2).

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.

Positive Control
CD46 is expressed on every cell and tissue

Negative Control

Product type
Monoclonal antibodies
1 mg, 10 mg, 100 µg, 20 µg, 5 mg, 500 µg
Quantities >500 µg will be send without preservative and carrier free. Quantities <500 µg will be send with preservative and carrier. exact formulation can be found on the datasheet.
Purified MCP
Mouse IgG1
Membrane cofactor protein, TLX, trophoblast leucocyte common antigen<br> - Gene name: CD46
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Infectious diseases, Tumor immunology

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