Home \ Antibodies \ CD46, Human, mAb M177
CD46, Human, mAb M177-0
CD46, Human, mAb M177-1217
CD46, Human, mAb M177-1216
CD46, Human, mAb M177-0CD46, Human, mAb M177-1217CD46, Human, mAb M177-1216
Catalog # HM2103

CD46, Human, mAb M177

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The monoclonal antibody M177 recognizes CD46, also designated membrane cofactor protein (MCP). CD46 is a 45-70 kDa protein with genetic and tissue-specific heterogeneity.- It is expressed on every cell and tissue, with the exception of erythrocytes. CD46 serves to inhibit complement activation on host tissue. It performs this function by serving as a cofactor which binds to C3b and C4b. This binding is permitted by factor I, a serine protease of plasma, to degrade C3b and C4b and serves to protect the host cell against autologous attack. It also serves as a receptor for measles virus.
Four isoforms of CD46 predominate and arise by alternative splicing of a single CD46 gene. CD46 cDNA encodes a signal sequence followed by four complement control protein domains (also called short consensus repeats (SCR)). The monoclonal antibody M177 reacts with the SCR2 domain.

Application
Flow cytometry, Frozen sections, Functional studies, Immuno assays, Immuno precipitation, Western blot

Application Notes
FC: Antibody M177 stains the extracellular domain of CD46.
IP: 107 cells were lysed and immunoprecipitated with 25 µg M177 antibody and 25 µg protein G-sepharose
W:A non-reduced sample treatment and SDS-page was used. The band size is 45-70 kDa (Ref 1 and 2).

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.

Positive Control
CD46 is expressed on every cell and tissue

Negative Control
Erythrocytes

Product type
Monoclonal antibodies
Amount
1 mg, 10 mg, 100 µg, 20 µg, 5 mg, 500 µg
Formulation
Quantities >500 µg will be send without preservative and carrier free. Quantities <500 µg will be send with preservative and carrier. exact formulation can be found on the datasheet.
Immunogen
Purified MCP
Isotype
Mouse IgG1
Species
Human
Alias
Membrane cofactor protein, TLX, trophoblast leucocyte common antigen<br> - Gene name: CD46
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease
Infectious diseases, Tumor immunology
Protocol
Protocol Western Blot
59 kb
Protocol SDS Page
57 kb
Protocol Sandwich ELISA
49 kb
Protocol Immuno precipitation
54 kb
Protocol Flow Cytometry
58 kb
CoA-TDS HM2100-HM2199
HM2103-100UG
130 kb
HM2103-10MG
130 kb
HM2103-1MG
130 kb
HM2103-20UG
130 kb
HM2103-500UG
130 kb
HM2103-5MG
130 kb
HM2103-IA
130 kb
HM2103F-100UG
130 kb
HM2103F-20UG
130 kb
Safety Data Sheet
SDS - HM2103-100UG
51 kb
SDS - HM2103-10MG
50 kb
SDS - HM2103-1MG
50 kb
SDS - HM2103-20UG
51 kb
SDS - HM2103-500UG
50 kb
SDS - HM2103-5MG
50 kb

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