The monoclonal antibody MM10 recognizes membrane cofactor protein (MCP), also known as CD46. MCP is a type I membrane glycoprotein that consists of four isoforms i.e. two C-isoforms (51-58 kDa) and two BC isoforms (59-68 kDa) which arise by alternative splicing of a single CD46 gene. Membrane cofactor protein is expressed on every cell and tissue, with the exception of erythrocytes. Membrane cofactor protein serves to down-regulate the activation of complement on host tissue. MCP is a cofactor for factor 1-mediated cleavage of C3b and C4b and this way serves to protect the host cell against autologous attack. MCP is a receptor for several pathogens including measles virus, group A Streptococcus pyogenes and pathogenic Neisseria.
Flow cytometry, Frozen sections, Immuno assays, Immuno fluorescence, Western blot
For immune histology, flow cytometry and western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilution. The typical starting working dilution is 1:50.
IA: Rat CD46 or mouse CD46 variant as coating on a micro titer plate