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Catalog # HM1118

CD46, Mouse, mAb MM10

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The monoclonal antibody MM10 recognizes membrane cofactor protein (MCP), also known as CD46. MCP is a type I membrane glycoprotein that consists of four isoforms i.e. two C-isoforms (51-58 kDa) and two BC isoforms (59-68 kDa) which arise by alternative splicing of a single CD46 gene. Membrane cofactor protein is expressed on every cell and tissue, with the exception of erythrocytes. Membrane cofactor protein serves to down-regulate the activation of complement on host tissue. MCP is a cofactor for factor 1-mediated cleavage of C3b and C4b and this way serves to protect the host cell against autologous attack. MCP is a receptor for several pathogens including measles virus, group A Streptococcus pyogenes and pathogenic Neisseria.

Application
Flow cytometry, Frozen sections, Immuno assays, Immuno fluorescence, Western blot

Use
For immune histology, flow cytometry and western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilution. The typical starting working dilution is 1:50.

Positive Control
IA: Rat CD46 or mouse CD46 variant as coating on a micro titer plate

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg
Formulation
0.2 ml (100 µ/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Immunogen
Mouse CD46-human Fc fusion protein
Isotype
Rat IgG2a
Species
Mouse
Cross reactivity
Rat – Yes
Alias
MCP, CD46, Trophoblast leukocyte common antigen, TLX, measles virus receptor
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Infectious diseases, Tumor immunology
Protocol
Protocol Western Blot
59 kb
Protocol SDS Page
57 kb
Protocol Sandwich ELISA
49 kb
Protocol Immuno Fluorescence
39 kb
Protocol Flow Cytometry
58 kb
Protocol IHC-F
36 kb
CoA-TDS HM1067-HM2099
HM1118-100UG
51 kb
HM1118-20UG
58 kb
Safety Data Sheet
SDS - HM1118-100UG
52 kb
SDS - HM1118-20UG
52 kb

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