Home \ Antibodies \ Complement factor I, Human, mAb 1C11
Catalog # HM2320

Complement factor I, Human, mAb 1C11

120,00375,00

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The monoclonal antibody 1C11 recognizes Complement Factor I (CFI). CFI is a protein of the complement system (serine protease) , also known as as C3b/C4f inactivator, and is a protein that is encoded by the CFI gene located on chromosome 4. It regulates complement activation by cleaving cellbound or fluid phase C3b and C4b. CFI is synthesised predominantly in the liver, and is initially secreted as a single 88 kDa gene product; this precursor protein is then cleaved by furin to yield the mature CFI protein, which is a disulfide-linked dimer of heavy chain residues (residue 19-335, 51 kDa) and light chain (residues 340-583, 37 kDa). Only the mature protein is active. Genetic polymorphism in CFI has been observed (variants R201S, R406H, R502L). CFI deficiency leads to low levels of complement component 3 (C3) in plasma, due to unregulated activation of the complement alternative pathway, and it has been associated with recurrent bacterial infections in children; more recently, mutations in the CFI gene have been shown to be implicated in development of Haemolytic Uremic Syndrome, a renal disease also caused by unregulated complement activation. The monoclonal antibody can be used to detect CFI levels in serum and plasma.

Application
Immuno assays, Western blot

Application Notes
WB: FI is a 88 kDa heterodimer cleaved out to yield the heavy (50 kDa) and the light (38 kDa) chains (Ref3) IA: The antibody is suitable as both a coat and tracer in immuno assays.

Use
For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
Quantity
20 µg, 100 µg
Formulation
0.2 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Immunogen
Complement factor I
Isotype
Mouse IgG1
Species
Human
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Infectious diseases, Nephrology
Protocol CoA-TDS HM2300-HM2400
HM2320
77 kb

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