Home \ Antibodies \ Complement factor I, Human, mAb MBI-1
Catalog # HM2319

Complement factor I, Human, mAb MBI-1

120,00375,00

Please choose the quantity of items you wish to add to your cart…

The monoclonal antibody MBI-1 recognizes Complement Factor I (CFI). CFI is a protein of the complement system (serine protease) , also known as as C3b/C4f inactivator, and is a protein that is encoded by the CFI gene, located on chromosome 4. It regulates complement activation by cleaving cell bound or fluid phase C3b and C4b. CFI is synthesized mostly in the liver, and is initially secreted as a single 88 kDa gene product; this precursor protein is then cleaved by furin to yield the mature CFI protein, which is a disulfide-linked dimer of heavy chain residues (residue 19-335, 51 kDa) and light chain (residues 340-583, 37 kDa). Only the mature protein is active. genetic polymorphism in CFI has been observed (variants R201S, R406H, R502L). CFI deficiency leads to low levels of complement component 3 (C3) in plasma, due to unregulated activation of the complement alternative pathway, and it has been associated with recurrent bacterial infections in children; more recently, mutations in the CFI gene have been shown to be implicated in development of Haemolytic Uremic Syndrome, a renal disease also caused by unregulated complement activation. The monoclonal antibody can be used to detect CFI levels in serum, plasma.

Application
Immuno assays, Western blot

Application Notes
W: A non-reduced and reduced sample treatment and SDS-Page was used. The band size is 66 kDa is samples are non-reduced. Reduced samples have a band size of 40 kDa. IA: the antibody can be used as coat and detector

Use
For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
Quantity
20 µg, 100 µg
Formulation
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Immunogen
Complement factor I
Isotype
Mouse IgG1
Species
Human
Alias
CFI
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Infectious diseases, Nephrology
CoA-TDS HM2300-HM2400
HM2319
77 kb

You may also like…

Calculate your ELISA data easily

With the ELISA calculator you can easily calculate ELISA data. Assayfit Pro helps to perform curve fitting. The calculator generates advanced reports, fit graph, fit parameters and goodness of fit are shown.

Hycultbiotech_contact us

Would you like some virtual coffee?

Contact us