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Catalog # HM2319

Complement factor I, Human, mAb MBI-1

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The monoclonal antibody MBI-1 recognizes Complement Factor I (CFI). CFI is a protein of the complement system (serine protease) , also known as as C3b/C4f inactivator, and is a protein that is encoded by the CFI gene, located on chromosome 4. It regulates complement activation by cleaving cell bound or fluid phase C3b and C4b. CFI is synthesized mostly in the liver, and is initially secreted as a single 88 kDa gene product; this precursor protein is then cleaved by furin to yield the mature CFI protein, which is a disulfide-linked dimer of heavy chain residues (residue 19-335, 51 kDa) and light chain (residues 340-583, 37 kDa). Only the mature protein is active. genetic polymorphism in CFI has been observed (variants R201S, R406H, R502L). CFI deficiency leads to low levels of complement component 3 (C3) in plasma, due to unregulated activation of the complement alternative pathway, and it has been associated with recurrent bacterial infections in children; more recently, mutations in the CFI gene have been shown to be implicated in development of Haemolytic Uremic Syndrome, a renal disease also caused by unregulated complement activation. The monoclonal antibody can be used to detect CFI levels in serum, plasma.

Immuno assays, Western blot

Application Notes
W: A non-reduced and reduced sample treatment and SDS-Page was used. The band size is 66 kDa is samples are non-reduced. Reduced samples have a band size of 40 kDa. IA: the antibody can be used as coat and detector

For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
100 µg, 20 µg
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Complement factor I
Mouse IgG1
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Infectious diseases, Nephrology
CoA-TDS HM2300-HM2400
77 kb
Safety Data Sheet

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