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Catalog # HM2105

DAF, Human, mAb D17

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CD55, also designated decay accelerating factor (DAF), is a 60-70 kDa glycosyl-phosphatidylinositol (GPI) anchored protein. CD55 is a member of the family of proteins that protect host tissue from damage by autologous complement. CD55 was first recognized as a species restricting factor operating at the level of C3 activation. It binds C3b and C4b to inhibit formation and half-life of the C3 convertases. CD55 expression increases upon T cell activation and in the presence of phorbol esters antibodies to CD55 (e.g. monoclonal antibody D17) are mitogenic. CD55 is broadly distributed among cells in contact with serum, including both haematopoietic and non-haematopoietic cells. Although CD55 does not have an essential role in controlling hemolysis of erythrocytes, it has an important role in regulation of the deposition of C3 on nucleated cells.

Application
Flow cytometry, Western blot

Use
For flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg
Formulation
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Isotype
Mouse IgG1
Species
Human
Alias
DAF (decay accelerating factor), CD55
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Autoimmunity, Infectious diseases
Protocol
Protocol Western Blot
59 kb
Protocol SDS Page
57 kb
Protocol Flow Cytometry
58 kb
CoA-TDS HM2100-HM2199
HM2105-100UG
47 kb
HM2105-20UG
47 kb
HM2105F-100UG
47 kb
HM2105F-20UG
47 kb
Safety Data Sheet
SDS - HM2105-100UG
52 kb
SDS - HM2105-20UG
52 kb

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