The monoclonal antibody BV12 recognizes junctional adhesion molecule-A (JAM-A), also known as JAM-1 and the mouse platelet F11-Receptor (F11R), is a cell adhesion molecule (CAM). JAM-A is a member of the immunoglobulin superfamily found on the surface of mouse platelets and at intercellular junctions of endothelial cells and epithelial cells. JAM-A belongs together with JAM-B (VE-JAM or JAM-3) and JAM-C (JAM-2) to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAM plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. In human JAM-A plays a role in platelet aggregation, secretion, adhesion and spreading.
Flow cytometry, Immuno fluorescence, Immuno precipitation, Western blot
The monoclonal antibody BV12 can be used for flow cytometry, Western blotting, immuno precipitation and immuno assays. The monoclonal antibody BV12 is also useful for immunofluorescence on fixed cells.
For flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.