MBL, Human, mAb 3E7

Name: MBL, Human, mAb 3E7
SKU: HM2061
Availability: InStock

€125.00 – €1,174.00

Please choose the quantity of items you wish to add to your cart…

Amount

Quantity:

Add to basket

Description
Application

Mannose Binding Lectin (MBL) also called mannose- or mannan-binding protein (MBP) is a member of the group of collectins. MBL is an oligomeric lectin that recognizes carbohydrates as mannose and N-acetylglucosamine on pathogens. MBL contains a cysteine rich, a collagen like and a carbohydrate recognition domain. It forms a complex with C1r/C1s like serine proteases designated MASPs that proteolytically cleave C4, C2 and C3. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways. The MBL-MASP pathway (better known as the lectin pathway) is antibody and C1q-independent. MBL exhibits complement-dependent antibacterial activity and acts directly as an opsonic and therefore plays an important role in innate immunity. MBL is synthesized by hepatocytes and has been isolated from the liver or serum of various vertebrate species.

Application

Flow cytometry, Frozen sections, Functional studies, Immuno assays

Application Notes

FC: cells were incubated with 1.0 μg/ml MBL in PBS containing 1%BSA (w/v), 0.01% NaN3 (w/v) and 10 mM CaCl2 for 30 min at 0°C. The cells were washed with the same buffer, and incubated with antibody clone 3E7, 6.5 μg/ml. After 30 min at 0°C, cells were washed and incubated with phycoerythrin (PE)-conjugated goat F(ab’)2 anti-mouse Ig (Ref.3).
IHC-F: Tissue sections embedded in ornithine carbamoyltransferase compound were frozen in acetone dry ice and immersed in Tris-buffered saline (TBS) three times for 5 minutes. Treatment in 0.5% hydrogen peroxide in methanol solution has been done to quench endogenous peroxidase activity (Ref.2).
FS: Pretreatment of sheep erythrocytes with antibody 3E7 suppressed hemolysis in a dose dependent manner (Ref.1).

Use

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.

Negative Control

Nonimmune normal serum or TBS

Specifications
Downloads

Product type

Monoclonal antibodies

Amount

100 µg, 20 µg, 500 µg

Formulation

Quantities >500 µg will be send without preservative and carrier free. Quantities <500 µg will be send with preservative and carrier. exact formulation can be found on the datasheet.

Immunogen

Serum isolated human RaRF

Isotype

Mouse IgG1

Species

Human

Alias

Mannan Binding Lectin, mannose- or mannan-binding protein, MBP

Storage and stability

Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.

Precautions

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.

Disease

Infectious diseases

CoA-TDS