MCP-1, Human, mAb MNA1

Name: MCP-1, Human, mAb MNA1
SKU: HM2011
Availability: InStock

€133.00 – €368.00

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Description
Application

Monoclonal antibody MNA.1 (formerly known as 5D3-F7) recognizes human natural and recombinant monocyte chemotactic protein-1 (MCP-1). Monocyte chemotactic protein-1 (MCP-1) is a 11 kDa protein belonging to the CC subgroup of the chemokine superfamily, which stimulate the migration of monocytic cells. In contrast, the CXC chemokines predominantly activate polymorphonuclear leukocytes. The coordinated synthesis and release of MCP-1 plays a central role in both acute and chronic inflammatory processes by controlling the influx of phagocytic cells. Furthermore, their state of activation is in concert with primary inflammatory cytokines, such as IL-1, TNF-a, and IL-6. A selective accumulation of MCP-1 in the cerebrospinal fluid (CSF) of AIDS patients with cytomegalovirus encephalitis, but not with other opportunistic infections or primary lymphomas of the central nervous system , has been described. Furthermore, the chemotactic activity of MCP-1 on monocytic cells has been suggested to play a role in psoriasis, rheumatoid arthritis and atherosclerosis. No cross-reactivity of mAb MNA.1 with other cytokines has been detected.

Application

Flow cytometry, Frozen sections, Functional studies, Immuno assays, Immuno precipitation, Paraffin sections, Western blot

Application Notes

FC: Antibody MNA.1 stains intracellular MCP-1.For intracellular staining HUVEC cells were permeabilized with buffer containing- 0.1% saponin. The cells were fixed in 4% paraformaldehyde before staining. As negative control a corresponding isotype control antibody was used- (Ref.5).
W: A reduced sample treatment and 15% SDS-Page was used. The band sizes are ~14 and 11 kDa (Ref.1).
IHC-P: Tissue sections were fixed in formalin and pretreated with hydrogen peroxide to quench endogenous peroxidases. Antigen retrieval was performed by pressure cooking for 3 minutes in PBS. As negative control the MNA.1 antibody was omitted (Ref.7).
IHC-F:- Tissue sections were air dried and pretreated with 3% hydrogen peroxide to quench endogenous peroxidases. As negative control the MNA.1 antibody was omitted (Ref.2).
FS: Antibody MNA.1 inhibits migration of monocytes by neutralizing MCP-1(Ref.1).

Use

For immunohistochemistry, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies- dilutions have to be optimized in user’s experimental setting.

Specifications
Downloads

Product type

Monoclonal antibodies

Amount

100 µg, 20 µg

Formulation

0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.

Immunogen

Recombinant human MCP-1

Isotype

Mouse IgG1

Species

Human

Cross reactivity

Pig – Yes

Alias

C-C motif chemokine 2, CCL2, small-inducible cytokine A2, MCAF, HC11

Storage and stability

Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Precautions

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.

Disease

Autoimmunity, Infectious diseases

Protocol