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Catalog # HM2011

MCP-1, Human, mAb MNA1

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Monoclonal antibody MNA.1 (formerly known as 5D3-F7) recognizes human natural and recombinant monocyte chemotactic protein-1 (MCP-1). Monocyte chemotactic protein-1 (MCP-1) is a 11 kDa protein belonging to the CC subgroup of the chemokine superfamily, which stimulate the migration of monocytic cells. In contrast, the CXC chemokines predominantly activate polymorphonuclear leukocytes. The coordinated synthesis and release of MCP-1 plays a central role in both acute and chronic inflammatory processes by controlling the influx of phagocytic cells. Furthermore, their state of activation is in concert with primary inflammatory cytokines, such as IL-1, TNF-a, and IL-6. A selective accumulation of MCP-1 in the cerebrospinal fluid (CSF) of AIDS patients with cytomegalovirus encephalitis, but not with other opportunistic infections or primary lymphomas of the central nervous system , has been described. Furthermore, the chemotactic activity of MCP-1 on monocytic cells has been suggested to play a role in psoriasis, rheumatoid arthritis and atherosclerosis. No cross-reactivity of mAb MNA.1 with other cytokines has been detected.

Application
Flow cytometry, Frozen sections, Functional studies, Immuno assays, Immuno precipitation, Paraffin sections, Western blot

Application Notes
FC: Antibody MNA.1 stains intracellular MCP-1.For intracellular staining HUVEC cells were permeabilized with buffer containing- 0.1% saponin. The cells were fixed in 4% paraformaldehyde before staining. As negative control a corresponding isotype control antibody was used- (Ref.5).
W: A reduced sample treatment and 15% SDS-Page was used. The band sizes are ~14 and 11 kDa (Ref.1).
IHC-P: Tissue sections were fixed in formalin and pretreated with hydrogen peroxide to quench endogenous peroxidases. Antigen retrieval was performed by pressure cooking for 3 minutes in PBS. As negative control the MNA.1 antibody was omitted (Ref.7).
IHC-F:- Tissue sections were air dried and pretreated with 3% hydrogen peroxide to quench endogenous peroxidases. As negative control the MNA.1 antibody was omitted (Ref.2).
FS: Antibody MNA.1 inhibits migration of monocytes by neutralizing MCP-1(Ref.1).

Use
For immunohistochemistry, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies- dilutions have to be optimized in user’s experimental setting.

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg
Formulation
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
Immunogen
Recombinant human MCP-1
Isotype
Mouse IgG1
Species
Human
Cross reactivity
Pig – Yes
Alias
C-C motif chemokine 2, CCL2, small-inducible cytokine A2, MCAF, HC11
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Autoimmunity, Infectious diseases
Protocol
Protocol Western Blot
59 kb
Protocol SDS Page
57 kb
Protocol Sandwich ELISA
49 kb
Protocol IHC-P
43 kb
Protocol Flow Cytometry
58 kb
Protocol IHC-F
36 kb
CoA-TDS HM1067-HM2099
HM2011-100UG
86 kb
HM2011-20UG
86 kb
Safety Data Sheet
SDS - HM2011-100UG
51 kb
SDS - HM2011-20UG
51 kb

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