Home \ Antibodies \ Properdin, Human, mAb 2.9
Catalog # HM2282

Properdin, Human, mAb 2.9

120,00333,00

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The monoclonal antibody 2.9 reacts with human properdin (factor P), which is a single-chain plasma
glycoprotein with an apparent molecular mass of 52-55 kDa, The protein domain structure of
properdin consists of six thrombospondin repeat sequences between short N- and C-terminal
domains. In blood, properdin exists as a mixture of head-to-tail dimers, trimers and tetramers. The
protein is expressed by a variety of leukocytes, including monocytes, T lymphocytes and neutrophils,
but also by endothelial cells in which properdin synthesis is induced by certain stress factors.
Properdin participates in the alternative pathway of complement activation together with C3 and
factors B, D, I and H by prolonging the half-life of the labile C3bBb, which is deposited on immune
complexes or foreign surfaces. This permits amplification of C3bBb formation in competition with
catabolism of C3b by factor I, which uses factor H as a cofactor. The local amplification process leads
to the creation of the alternative pathway C5 convertase, C3bBb3b, and initiates the terminal pathway
of complement activation. As a consequence, properdin is consumed by binding to C3bBb, which
shows an order of preference of tetramers over trimers over dimers, which corresponds to the
functional activity of the oligomeric forms. Deficiency or malfunction of the molecule may lead to
severe impairment of alternative pathway activation, depending on the precise nature of the defect.
Three types of deficiencies have been described so far: type 1 (or I) is characterized by serum with
very low or absent properdin activity in hemolytic assays and <0.1 μg/ml immunoreactive protein; type 2 (or II) is characterized by low but detectable levels of immunoreactive protein (>2 μg/ml) and
impairment of some, but not all functional test, and type 3 (or III) has normal levels of immunoreactive
but dysfunctional protein (5-25 μg/mL). Lower properdin levels were found in 70% of diabetic patients
when compared to nondiabetic controls and is suggested by the authors that patients with low
expression of properdin take preventive measures and early treatments against infection. The
monoclonal antibody 2.9 is suitable for functional studies as well as use in immunoassays.

Application
Immuno assays

Use
For Immuno Assays dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.

Product type
Monoclonal antibodies
Quantity
20 µg, 100 µg
Formulation
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin albumin and 0.02% sodium azide..
Immunogen
Purified human Properdin
Isotype
Mouse IgG2a
Species
Human
Alias
Factor P
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Autoimmunity
Protocol CoA-TDS HM2200 - 2300
HM2282
75 kb

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