Home \ Antibodies \ S100A12, Human, mAb 2
W: 0.25 µg recombinant S100A12 was loaded. The concentrstion of HM2361 used was 2 µg/ml.
Catalog # HM2361

S100A12, Human, mAb 2

120,001.022,00

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The monoclonal antibody clone 2 recognizes S100 calcium-binding protein A12 (S100A12). The antibody does not cross-react with family members S100A7, S100A8 and S100A9, S100A8/A9 heterodimers and S100P. S100A12, also known as calgranulin C, is a member of the S100 protein family. To date, the family consists of 22 members. S100 proteins are low molecular weight calcium binding proteins. The proteins consist of 2 calcium binding EF-hands located on the termini flanked by hydrophobic hinge regions. Calcium ions acts as a global second messenger involved in the regulation of many aspects of cell function. S100 proteins are involved in sensing and transforming calcium signals to downstream cellular responses. S100 proteins are located in the cytoplasm and/or nucleus of a wide range of cell types and involved in the regulation of cellular processes such as cell cycle progression and differentiation and inflammatory responses. Several S100 members have been found to act as cytokines in inflammation, particularly in autoimmune skin conditions such as psoriasis. S100A12 is involved in calcium-dependent signal transduction pathways and has regulatory effects on cytoskeletal components thereby modulating neutrophil activities. Studies have shown that S100A12 might have potential as a biomarker for arthritis since their levels correlate with disease activity and are able to predict relapse and response to anti-inflammatory therapy. Unfortunately, studying the biology of S100A12 and other calgranulins is challenging due to its tendency to form complex homodimer, heterodimer and heterotetramers structures.

Application
Immuno assays, Western blot

Application Notes
- IA: Antibody clone 2 can be used as capture and detection antibody.
- W: A reduced sample treatment and SDS-Page was used. The band size is ~11 kDa.
- No cross reactivity with S100A8, S100A9, S100A8/A9 and S100P

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
Quantity
20 µg, 100 µg, 500 µg
Formulation
Quantities >500 µg will be send without preservative and carrier free. Quantities <500 µg will be send with preservative and carrier. exact formulation can be found on the datasheet.
Immunogen
Recombinant S100A12
Isotype
Mouse IgG1
Species
Human
Alias
Protein S100-A12, CGRP, Calcium-binding protein in amniotic fluid 1, CAAF1, Calgranulin-C, CAGC, Extracellular newly identified RAGE-binding protein, EN-RAGE, Migration inhibitory factor-related protein 6, MRP-6, p6, Neutrophil S100 protein, S100 calcium-binding protein A12</br> - Gene name: S100A12
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Autoimmunity
CoA-TDS HM2300-HM2400
HM2361
67 kb

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