Antibody 2D9 recognizes human S100A7 (Psoriasin). S100A7 is a 11.4kDa member of the S100 gene family, which are EF-hand Ca 2+-binding proteins. They are involved in a broad range of intra- and extracellular processes like regulation of calcium homeostasis, cell proliferation, differentiation, apoptosis, immune responses, antimicrobial activity, chemotaxis and inflammation. S100 proteins have a fundamental structural unit which is a highly integrated dimer, some as homodimer and others as heterodimer. S100A7 functions as a homodimer and binds two Zn2+ ions. These ions stabilize the dimer and mediate the function of S100A7 during infection. Extracellular S100A7 functions as a DAMP during infection. S100A7 limits bacterial infection through metal limitation. Furthermore, S100A7interacts with RAGE which drives an NF-B mediated proinflammatory response and recruitment of neutrophils.
S100A7 was first discovered as a protein abundantly expressed in psoriatic keratinocytes. Possibly, S100A7 adheres directly to and reduces survival of pathogens found on the epidermis and acts as a physical barrier. S100A7 also seems to be involved with breast cancer and squamous cell carcinoma. In vitro it was found to confer a more aggressive behavior
Frozen sections, Functional studies, Immuno assays, Immuno fluorescence, Paraffin sections, Western blot
- W: A reduced sample treatment and SDS-Page was used. The band size is ~12 kDa.
- FS: Antibody clone 2D9 can be used as blocking antibody.
- IA: Antibody 2D9 can be used as detection antibody.
- No cross reactivity with S100A4, S100A8, S100A9, S100A11, S100P, S100B
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.