Podoplanin, Human, clone LpMab17

Quantity:
20 µg
Catalog #:
HM2375-20UG

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Monoclonal antibody LpMab17 recognizes human Podoplanin (PDPN; agrrus). Podoplanin is a small type_I transmembrane sialoglycoprotein expressed on a broad range of cell types and involved in platelet aggregation and tumor metastasis. The 36 kDa protein was initially identified as a biomarker of lympatic endothelium, alveolar epithelium and glomerular podocytes. Expression has also been found in many tumors. However the functional role of PDPN is still poorly understood. Podoplanin consists of an extracellular domain, transmembrane domain and a cytoplasmic tail. In its extracellular domain it possesses a platelet aggregation stimulating (PLAG) domain. Podoplanin has got three PLAG domains of which PLAG3 is critical for binding with C-type lectin receptor-2 (CLEC-2). This PDPN specific receptor mediates platelet aggregation. This interaction is required to initiate and maintain separation of blood and lymphatic vessels and can be critical in process of cancer metastasis. This may lead to PDPN as possible therapeutic target. Antibody LpMab-17 recognizes a non-PLAg domain (Gly77-Asp82). Although PDPN is quite conserved between species, LpMab17 does not bind monkey PDPN.
Specifications
Product type Monoclonal antibodies
Quantity 20 µg
Formulation 0.2 ml (100 µg/ml) 0.2 µm filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02 % sodium azide.
Application Flow cytometry, Immuno assays, Immuno fluorescence, Paraffin sections, Western blot
Immunogen LN229/hPDPN (Ref.1)
Isotype Mouse IgG1
Species Human
Alias Aggrus, Glycoprotein 36, Gp36, PA2.26 antigen, T1-alpha, T1A
References 1. Kato, Y et al; LpMab-12 Established by CasMab Technology Specifically Detects Sialylated O-Glycan on Thr52 of Platelet Aggregation-Stimulating Domain of Human Podoplanin. PlosOne 2016, 11: e0152912
2. Kaneko, M et al; Development and characterization of anti-glycopeptide monoclonal antibodies against human podoplanin, using glycan-deficient cell lines generated by CRISPR/Cas9 and TALEN. Cancer Medicine 2017, 6:382
Storage and stability Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Applications
Application: Flow cytometry, Immuno assays, Immuno fluorescence, Paraffin sections, Western blot
Application notes: IA: Antibody LpMab17 can be used as detection antibody
. FC: HM2375 can be used both intra- and extracellular.
W: reduced and non-reduced conditions were used. The expected band sizes are ~40 kDa.
References
References: 1. Kato, Y et al; LpMab-12 Established by CasMab Technology Specifically Detects Sialylated O-Glycan on Thr52 of Platelet Aggregation-Stimulating Domain of Human Podoplanin. PlosOne 2016, 11: e0152912
2. Kaneko, M et al; Development and characterization of anti-glycopeptide monoclonal antibodies against human podoplanin, using glycan-deficient cell lines generated by CRISPR/Cas9 and TALEN. Cancer Medicine 2017, 6:382
CoA-TDS HM2300-HM2400
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