Antimicrobial peptides in obstructive uropathy

Summary of Gupta, S. et al; Urinary antimicrobial peptides: potential novel biomarkers of obstructive uropathy. Journal of Pediatric Urology 2018. Click here for the full article.

Antimicrobial peptides (AMPs) have historically been evaluated for their role in protecting against uropathogens. However, AMPs have not been evaluated for expression in noninfectious urological injury such as urinary tract obstruction. The objective of this pilot study is to compare urinary AMP expression in children undergoing surgical intervention for ureteropelvic junction obstruction (UPJO) with non-obstructed controls.

Thirty bladder urine samples were obtained from children with UPJO and compared with matched controls on AMP levels of LL-37 (Cat. # HK321), NGAL (Cat. # HK330), HIP/PAP, BD-1 and HD-5. The results demonstrated that urinary AMP levels were significantly higher in UPJO patients than controls (NGAL and LL-37 both with a P-value of <0.001). The biological significance of increased urinary AMP levels in children with urinary tract obstruction remains unclear, but it is likely a reflection of the urothelial and tubular stress resulting from obstructive uropathy. It may further represent an adaptive mechanism of uroepithelial barrier function or protect against infection during urinary stasis. However, this should be confirmed by future studies on AMP expression as a biomarker of uroepithelial injury.

Graph showing NGAL levels in healthy controls and patients with uropathy