Basophils, Human, mAb 2D7

Name: Basophils, Human, mAb 2D7
SKU: HM2279
Availability: InStock

€133.00 – €368.00

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Description
Application

Unlock the secrets of immune response with our precision-targeted 2D7 monoclonal antibody, the definitive marker for basophils. This innovative tool zeroes in on a unique 7.2-7.5 kDa protein exclusive to basophil secretory granules, enabling researchers to track basophil activation with unparalleled clarity. As gatekeepers of inflammatory and allergic reactions, basophils are pivotal in the release of histamine and cytokines, and in prompting B cells to produce IgE. Originating in the bone marrow, these critical cells circulate in the bloodstream, ready to converge at inflammation sites. With the 2D7 antibody, detect the subtle interplay of basophils in allergic conditions with exacting specificity—excluding interference from lymphocytes, monocytes, eosinophils, neutrophils, and mast cells. Elevate your research with the precision of 2D7 antibody in immunohistochemistry and western blot applications, and illuminate the role of basophils in human health and disease.

Application

Flow cytometry, Frozen sections, Immuno fluorescence, Paraffin sections, Western blot

Application Notes

IF: Cytospin preparations were fixed in methanol for 15 min, incubated in methanol containing 0.6% H2O2, for 30 min to block endogenous peroxidase, washed in dH2O, incubated with goat serum (1/500 dilution) for 2 h to block nonspecific staining. Blocked slides were washed with TTBS, pH7.4. (Ref.1)
FC: Antibody 2D7 stains the extracellular domain of basophils. As negative control CD203c negative cells (basophil depleted cell fractions) (Ref.3)
W: A reduced sample treatment and SDS-Page was used. The band sizes are 72 en 76 kDa (Ref.1).
IHC-P: Fresh surgical tissues were fixed in Carnoy's fluid for 24 h and transferred to absolute ethanol. Tissues were embedded in paraffin, and 4- µm sections were prepared. Tissue sections were dewaxed in xylene and rehydrated in graded ethanol solutions. Endogenous peroxidase was inhibited. (Ref.1)
IHC-F: Sections were fixed in either 10% NBF or Carnoy's fluid for 15 minutes at RT. NBF-fixed tissue sections were digested with 0.1% protease in TTBS for 20 min at RT. Endogenous peroxidase activity was inhibited by methanol with 0.6% H2O2 for 30 min at RT. Blocked by incubation in horse serum at 1:20 dilution in TTBS for 1 hr at RT. 2D7 at a 1:300 dilution in TTBS overnight at 4 ° C or with a murine mAb of undetermined specificity (MOPC31-C) as a negative control. (Ref.2)

Use

For immunohistochemistry, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.

Positive Control

Chronic myeloid leukaemia (basophilia)

Specifications
Downloads

Product type

Monoclonal antibodies

Amount

100 µg, 20 µg

Formulation

0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.

Immunogen

Lysate of purified basophils

Isotype

Mouse IgG1

Species

Human

Storage and stability

Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label

Precautions

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.

Disease

Infectious diseases

Protocol