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Catalog # HM2412

C1s, Human, clone 10/12

Antibody 10/12 recognizes human activated C1s, a molecule of the complement C1 complex and hence part of the component that initializes classical pathway activation.

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Antibody 10/12 recognizes human activated C1s, a molecule of the complement C1 complex and hence part of the component that initializes classical pathway activation.

Upon recognition of immune complexes by C1q, C1r is autoactivated and subsequently activates C1s, which is then able to cleave C4 and C2, leading to the formation of the C3 convertase and further activation of the terminal pathway of the complement system. C1s deficiencies are associated with impaired function of the classical complement pathway.

The zymogen form of C1s is a glycosylated single chain polypeptide with a MW of around 85kDa and the domain architecture CUB1-EGF-CUB2-CCP1-CCP2-SP, while activation through C1r leads to a 58kDa and a 27kDa chain, linked by disulfide bonds. Monoclonal antibody 10/12 is a murine IgG1 antibody directed against the CCP1-CCP2-SP domains of activated C1s and can detect human C1s in immunoassays.

Functional studies, Immuno assays, Western blot

Application Notes
IA: Antibody 10/12 can be used as detection antibody.
W: Antibody 10/12 recognizes only the complex C1s-C1INH in western blot, both under reduced and non-reduced conditions. The expected band size is 150 - 196 kDa.

Product type
Monoclonal antibodies
100 µg, 20 µg
0.2 µm filtered in TBS+0.1%BSA
Recombinant C1s catalytic fragment, containing non-glycosylated, renatured form of CCP1-CCP2-SP domains, produced in E.Coli
Mouse IgG1
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
CoA-TDS Safety Data Sheet

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