The antibody reacts with human native and recombinant IL-6 as assessed by ELISA. The antibody inhibits the biological activity of human native and recombinant IL-6 as determined with the B9 cell bioassay. The antibody cross reacts with rhesus and cynomolgus natural IL6.
Functional studies, Immuno assays, Immuno precipitation, Western blot
For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions, starting with undiluted material. For neutralization of biological activity in vitro dilutions have to be made according to the amount of IL-6 to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.