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Catalog # HM4013

Lactoferrin, Bovine, mAb a-bC-lobe

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Monoclonal antibody a-bC-lobe, anti bovine Lactoferrin (Lf) is highly specific for bovine Lactoferrin. This protein is a member of the transferrin family of metal-binding proteins found in milk and other secretory fluids and also in blood. It shows multifunctional properties of which the bacteriostatic and bactericidal effects are the best known. The molecule is constructed with a N-terminal half molecule (N-lobe) and a C-terminal half molecule (C-lobe), each of which is composed of two domains. The biologically important functions have been found mainly in the N-lobe. The lactoferrin determinants responsible for binding to Ca2+-dependent receptor on hepatocytes are present within the C-lobe. The monoclonal antibody a-bC-lobe shows strong reactivities with both native and denatured forms of bovine lactoferrin and C-lobe. The ‘WNIPMGL’ sequence (467-473 of bovine lactoferrin) is the antigenic determinant or epitopic site of the anti C-lobe antibody a-bC-lobe. The antibody shows weak reactivity with human lactoferrin and korean goat lactoferrin, slight cross reactivity is seen with bovine transferrin, whereas no cross reactivity is seen with human transferrin and chicken ovotransferrin.

Immuno assays, Paraffin sections, Western blot

For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Product type
Monoclonal antibodies
100 µg, 20 µg
200 µl (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Mouse IgG1
Cross reactivity
Human – Yes
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Autoimmunity, Infectious diseases
Protocol CoA-TDS HM4000-6000 Safety Data Sheet

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