Home \ Antibodies \ Lactoferrin, Mouse, mAb 1E4
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Catalog # HM1133

Lactoferrin, Mouse, mAb 1E4

120,00375,00

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Monoclonal antibody clone 1E4 recognizes mouse lactoferrin (LF). The expression of small proteins and peptides with microbial activity, also called antimicrobial peptides (AMPs), is considered to be a primitive mechanism of immunity. AMPs are produced by almost all cell types which are commonly
exposed to microbes. Lactoferrin is a multifunctional AMP and a major element of innate immunity but also affects adaptive immune functions. LF is a well conserved mammalian non-haem iron-binding 80kD monomeric glycoprotein of the transferrin family. The protein is found in mucosal secretion like saliva, tears, urine, vaginal fluids, semen, urine and very high concentrations in (colostral) milk (1-7mg/ml). Its protective effects range from direct antimicrobial activities against a large panel of microorganisms, including bacteria, viruses, fungi and parasites, to anti-inflammatory and anticancer activities. The main functions of LF resided in specific domains and have predominantly be found in the N-terminal side. One of the main functions is inhibition of microbial growth by sequestration of iron, which is essential for growth. The N-terminal structure of LF is mainly responsible for this function. The N-terminal domain contains also a serine- protease-like activity which is involved in inactivation of host cell invasion by bacteria. Following infection, LF is released from secondary granules of
neutrophils in blood and inflamed tissue and functions as pattern-recognition receptor. Therefore, LF plasma concentration represents a positive relation to the total pool of neutrophils and the rate of neutrophil turnover and can be used as an index for neutrophil activation. In the adaptive immune response, LF promotes the maturation of T-cell precursors into competent helper cells and by
differentiation of immature B-cells into antigen presenting cells.

Application
Immuno assays, Western blot

Application Notes
IA:HM1133 can be used as capture antibody
W: A non-reduced and reduced sample treatment and SDS-page was used. The band size is 80 kDa.

Use
For Immuno Assay- dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.

Product type
Monoclonal antibodies
Quantity
20 µg, 100 µg
Formulation
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Immunogen
Mouse lactoferrin peptide
Isotype
Mouse IgG2b
Species
Mouse
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Autoimmunity, Infectious diseases
Protocol CoA-TDS HM1067-HM2099

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