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MASP-1, Human, clone 3/3-0
Catalog # HM2408

MASP-1, Human, clone 3/3

The anti MASP-1 monoclonal antibody clone 3/3 recognize the unique MASP-1 serine protease (SP) domain.

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The anti MASP-1 monoclonal antibody clone 3/3 recognize the unique MASP-1 serine protease (SP) domain. Mannan-binding lectin serine protease 1 or mannose-associated serine protease 1 (MASP-1) is an enzyme that is encoded by the MASP1 gene. Three types of human MASP have been reported, MASP-1, MASP-2 and MASP-3. MASP-1 is a serine protease that functions as a component of the lectin pathway of the complement system. The lectin pathway can be activated by the pathogen recognition receptors (PRRs) collectins (such as MBL and collectin kidney 1 (CL-K1 or CL-11) or by ficolins (ficolin-1 or M-ficolin, ficolin-2 or L-ficolin, and ficolin-3 or H-ficolin). The PRRs recognize carbohydrate and acetylated residues on the surface of pathogens and altered cells. After binding, MASP-1 zymogen auto activates and trans activates MASP-2. MASP-2 further cleaves the C4 complement component, whereas both MASP-1 and MASP-2 cleave C2. This leads to the formation of the C3 convertase (C4bC2b). The C3 convertase cleaves C3 leading to complement terminal pathway activation and thus mediating the destruction of infectious agents.

Application
Immuno assays, Western blot

Application Notes
- W: reduced and non-reduced western blot was performed. The expected band size for reduced is 15 and 30 kDa and for non-reduced 45 kDa. Longer exposure also shows a band at ~150 kDa, which is MASP-1 in complex.
- IA: HM2408 can be used as capture antibody.
- FS: C3 deposition was not inhibited with antibody HM2408 in an experiment with lectin pathway activation with c3 deposition measurement.

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg
Formulation
0.2 μm filtered in PBS+0.1%BSA+0.02%NaN3
Immunogen
Recombinant MASP-1 catalytic fragment, containing non-glycosylated, renatured form of CCP1-CCP2-SP domains, produced in E.Coli
Isotype
Mouse IgG1
Species
Human
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to - comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent - infringements that might result from the use or derivation of this product.
Disease
Infectious diseases, Nephrology
CoA-TDS HM2300-HM2400
HM2408-100UG
92 kb
Safety Data Sheet
SDS - HM2408-100UG
52 kb
SDS - HM2408-20UG
52 kb

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