The anti MASP-1 monoclonal antibody clone 3/3 recognize the unique MASP-1 serine protease (SP) domain. Mannan-binding lectin serine protease 1 or mannose-associated serine protease 1 (MASP-1) is an enzyme that is encoded by the MASP1 gene. Three types of human MASP have been reported, MASP-1, MASP-2 and MASP-3. MASP-1 is a serine protease that functions as a component of the lectin pathway of the complement system. The lectin pathway can be activated by the pathogen recognition receptors (PRRs) collectins (such as MBL and collectin kidney 1 (CL-K1 or CL-11) or by ficolins (ficolin-1 or M-ficolin, ficolin-2 or L-ficolin, and ficolin-3 or H-ficolin). The PRRs recognize carbohydrate and acetylated residues on the surface of pathogens and altered cells. After binding, MASP-1 zymogen auto activates and trans activates MASP-2. MASP-2 further cleaves the C4 complement component, whereas both MASP-1 and MASP-2 cleave C2. This leads to the formation of the C3 convertase (C4bC2b). The C3 convertase cleaves C3 leading to complement terminal pathway activation and thus mediating the destruction of infectious agents.
Immuno assays, Western blot
- W: reduced and non-reduced western blot was performed. The expected band size for reduced is 15 and 30 kDa and for non-reduced 45 kDa. Longer exposure also shows a band at ~150 kDa, which is MASP-1 in complex.
- IA: HM2408 can be used as capture antibody.
- FS: C3 deposition was not inhibited with antibody HM2408 in an experiment with lectin pathway activation with c3 deposition measurement.
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.