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Catalog # HP9047

Occludin, Human, pAb

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The polyclonal antibody reacts with human occludin, an integral membrane protein specifically associated with tight junctions. These specialized regions of the plasma membrane prevent the diffusion of dissolved molecules between adjacent epithelial cells, seal off body cavities such as intestine or stomach lumen and prevent the diffusion of membrane proteins and glycolipids between the apical and basolateral regions of the plasma membrane. Occludin is localized at tight junctions of both epithelial and endothelial cells and is highly expressed in kidney and has not been detected in testis. On the cytoplasmic surface of occludin, a domain within the C-terminal 150 residues has been shown to bind directly to the well-characterized tight junction protein zona occludens 1 (ZO-1). The antibody is generated against recombinant human occludin (aa 358-504). The polyclonal anti-human occludin antibody cross reacts with rat, mouse and dog occludin.

Application
Frozen sections, Immuno fluorescence, Western blot

Use
For immunohistology, immunfluorescence and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50..

Product type
Polyclonal antibodies
Amount
100 µg, 20 µg
Formulation
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Isotype
Guinea pig Ig
Species
Human
Cross reactivity
Canine – Yes, Mouse – Yes, Rat – Yes
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease
Tumor immunology
Protocol
Protocol Western Blot
59 kb
Protocol SDS Page
57 kb
Protocol Immuno Fluorescence
39 kb
Protocol IHC-F
36 kb
CoA-TDS pAb
HP9047
48 kb
HP9047-20UG
48 kb
Safety Data Sheet
SDS - HP9047-100UG
52 kb
SDS - HP9047-20UG
52 kb

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