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Catalog # HM2354

Properdin, Human, mAb 1G6D2


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Monoclonal antibody 1G6D2 recognizes human properdin, also called complement factor P. The complement system is the first line of defense against pathogens and facilitates elimination of apoptotic and damaged cells. Positive regulator plasma protein properdin is critical for the alternative pathway of complement. It is a single-chain glycoprotein (ca 53kDa) consisting of six TSR sequences. In the blood it exists as a mixture of preferably head-to-tail trimers, but also dimers and tetramers. The protein is produced by leukocytes, like activated neutrophils monocytes and T-lymphocytes, but also by eg. stressed endothelial cells.
Properdin can both initiate and positively regulate the alternative pathway activity together with C3 and factors B, D, I and H. It binds to C3b where It stabilizes the labile C3bBb convertase which is deposited on immune complexes or foreign surfaces. Thereby enhancing the AP by stimulation of amplification of C3bBb-convertase formation in competition with catabolism of C3b by factor I, which uses factor H as a cofactor. The local amplification process leads to the creation of the alternative pathway C5 convertase, C3bBb3b, and initiates the terminal pathway of complement activation. The alternative pathway may account for ca 80% of the terminal pathway activity. Properdin has also been shown to directly limit factor H activity.
Recent studies show that properdin is also a pattern-recognition receptor (PRR) able to bind directly to microbial surfaces, apoptotic and necrotic cells (dangerous nonself and altered self). Inappropriate activation or dysregulation of the alternative pathway is a critical factor in development of several autoimmune conditions. Targets opsonized with properdin are labeled for clearance by scavenger cells, even without complement. This makes it a potential therapeutic target in diseases. Recent studies has shown renewed interest in the evaluating role of properdin in disease pathogenesis, like Asthma, arthritis, septic shock, AMD and C3 glomerulopathy.
Antibody 1G6D2 can be used in ELISA, western blotting (only non-reduced) and as negative control antibody for functional studies inhibiting the alternative pathway of complement (Ref 1).

Functional studies, Immuno assays, Western blot

Application Notes
W: A non-reduced sample treatment was used. The band size is 52 kDa.
FS: Inhibition of the alternative complement pathway tested with HM2354 as a control for not inhibiting and HM2355 as an inhibition antibody. Different concentrations have been tested (0 – 5 μg/ml).

Positive Control
HM2355: Properdin, Human, clone 3A3E1

Negative Control
HM2354: Properdin, Human, clone 1G6D2

Product type
Monoclonal antibodies
100 µg, 20 µg, 500 µg
Quantities >500 µg will be send without preservative and carrier free. Quantities <500 µg will be send with preservative and carrier. exact formulation can be found on the datasheet.
Human properdin purified from human plasma.
Mouse IgG1
Complement factor P<br> - Gene name: CFP, PFC
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Autoimmunity, Nephrology
CoA-TDS HM2300-HM2400
87 kb
CoA-TDS HM2200 - 2300 Safety Data Sheet

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