MASP-2, Human, ELISA kit

Please notice Hycult Biotech cannot guarantee any batch-to-batch variability for this specific kit. We advise to use the same lot number if multiple kits are needed for a larger project. For more information or larger quantities please contact your distributor or support@hycultbiotech.com.
Quantity:
1 x 96 det.
Catalog #:
HK326-01

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MASP-2 is a MBL associated serine protease. Mannan-binding lectin (MBL) and ficolins, in complex with MASPs, are capable of activating the complement system, thus mediating the destruction of infectious agents. After oligomerisation of MBL (or L-Ficolin) MASP-2 will be bound and activated. Activated MASP-2 cleaves C4 and C2 and is crucial for the activation of downstream complement components. MASP-2 deficiency will result in an increased susceptibility for infections (e.g. leukemia patients in chemotherapy are at high risk of serious infections). Even more so then MBL deficiency, because MASP-2 is also required for complement activation by H- and L-ficolin. The ELISA detects MASP-2 in serum and plasma. The MASP-2 levels in plasma from healthy individuals range from 170 to 1196 ng/ml.
Specifications
Product type Assays
Quantity 1 x 96 det.
Standard range 1.6-100 ng/ml
Detection level 1.6 ng/ml
Working volume 100 µl/well
Species Human
Application The human MASP-2 ELISA kit is to be used for the in vitro quantitative determination of human MASP-2 in serum, plasma and cell culture supernatant samples.
Principle The human MASP-2 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle which a working time of 3½ hours. The efficient format of 2 plates with twelve disposable 8-well strips allow free choice of batch size for the assay. Samples and standards are captured by a solid bound specific antibody. Biotinylated tracer antibody will bind to captured human MASP-2. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human MASP-2 standards (log). The human MASP-2 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
Precautions For research use only. The kit is to be used for research purposes only, not for clinical or diagnostic use. The MASP-2 ELISA is Patent protected by patent 09/874,238 and PCT/DK2004/000338. License rights on the Patents are acquired from Natimmune S/A, Denmark. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
References 1. Møller-Kritensen, M et al; Levels of mannan-binding lectin-associated serine protease-2 in healthy individuals. J Immunol Meth 2003, 282: 159
2. Schlapbach, L et al; Deficiency of mannose-binding lectin-associated serine protease-2 associated with increase risk of fever and neutropenia in pediatric cancer patients. Ped Infect Dis 2007, 26: 989
3. Garcia-Laorden, M et al; Mannose-binding lectin and mannose-binding lectin–associated serine protease 2 in susceptibility, severity, and outcome of pneumonia in adults. J Allergy Clin Immunol 2008, 122: 368
4. Zehnder, A et al; Prognosis in pediatric hematologic malignancies is associated with serum concentration of mannose-binding lectin-associated serine protease-2 (MASP-2). Pediatr Blood Cancer 2009, 53: 53
5. Schlapbach, L et al; Higher Cord Blood Levels of Mannose-Binding Lectin-Associated Serine Protease-2 in Infants with Necrotising Enterocolitis. Pediatric Res 2008, 64: 562
Disease Infectious diseases
Applications
Application assays: The human MASP-2 ELISA kit is to be used for the in vitro quantitative determination of human MASP-2 in serum, plasma and cell culture supernatant samples.
Principle: The human MASP-2 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle which a working time of 3½ hours. The efficient format of 2 plates with twelve disposable 8-well strips allow free choice of batch size for the assay. Samples and standards are captured by a solid bound specific antibody. Biotinylated tracer antibody will bind to captured human MASP-2. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human MASP-2 standards (log). The human MASP-2 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.
References
Perfomance: The linearity of the assay was determined by serially diluting a sample containing 328 ng/ml human MASP-2. The diluted samples were measured in the assay. The line obtained a slope of 1.01 and a correlation coefficient of 0.999.
References
References: 1. Møller-Kritensen, M et al; Levels of mannan-binding lectin-associated serine protease-2 in healthy individuals. J Immunol Meth 2003, 282: 159
2. Schlapbach, L et al; Deficiency of mannose-binding lectin-associated serine protease-2 associated with increase risk of fever and neutropenia in pediatric cancer patients. Ped Infect Dis 2007, 26: 989
3. Garcia-Laorden, M et al; Mannose-binding lectin and mannose-binding lectin–associated serine protease 2 in susceptibility, severity, and outcome of pneumonia in adults. J Allergy Clin Immunol 2008, 122: 368
4. Zehnder, A et al; Prognosis in pediatric hematologic malignancies is associated with serum concentration of mannose-binding lectin-associated serine protease-2 (MASP-2). Pediatr Blood Cancer 2009, 53: 53
5. Schlapbach, L et al; Higher Cord Blood Levels of Mannose-Binding Lectin-Associated Serine Protease-2 in Infants with Necrotising Enterocolitis. Pediatric Res 2008, 64: 562
Assay Manual
pdf
(Size: 0.5 MB)
Safety Data Sheet
pdf
(Size: 0.14 MB)
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