Home \ Antibodies \ Factor Ba neoepitope, Human, mAb D22/3
Catalog # HM2379

Factor Ba neoepitope, Human, mAb D22/3

Monoclonal antibody D22/3 precisely targets human complement factor Ba (fBa), revealing its epitope even in denatured factor B during SDS-PAGE or western blotting. Its specificity to fBa in solution ensures reliable detection in complex biological contexts.

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Monoclonal antibody D22/3 actively targets human complement factor Ba (fBa), playing a pivotal role in the innate immune defense against pathogens. It engages factor Ba at the heart of the alternative pathway (AP) of complement activation, emerging from complement factor B (fB) cleavage by factor D in the presence of Mg2+. This cleavage generates Ba and Bb, positioning Ba as a key attractor of neutrophils and macrophages and a modulator of B cell function. Significantly, high Ba levels in chronic renal disease could signal a compromised immune response.

The D22/3 antibody distinctly recognizes the 234 amino acid glycoprotein Ba, formulated against a synthetic peptide from Ba’s carboxy terminus. It’s important to recognize that the epitope might become exposed in intact factor B under denaturing conditions, such as in SDS-PAGE or western blotting. However, in the fluid phase, D22/3’s precise specificity to Ba stands out, making it a crucial resource for complement research and a potential tool in understanding immune and renal disease mechanisms.

Immuno assays, Western blot

Application Notes
IA: HM2379 can be used as capture and detection antibody.

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
100 µg, 20 µg
0.2 µm filtered in PBS+0.02%NaN3+0.1%BSA
Synthesized C-terminal peptide Ba-20C (amino acid single letter code CETIEGVDAEDGHGPGEQQKR).
Mouse IgG2b
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
CoA-TDS Safety Data Sheet

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