FHR-2, Human, ELISA kit

Name: FHR-2, Human, ELISA kit
SKU: HK3004
Availability: InStock

The human FHR-2 ELISA kit is to be used for the in vitro quantitative determination of FHR-2 in serum and plasma samples.

€858.00 €1,413.00Price range: €858.00 through €1,413.00

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Description
Application

This ELISA is used for the quatification of protein Factor H-related 2 in serum and plasma samples.

Complement Factor H-related protein 2 (FHR-2) plays an important role in complement regulation. Predominantly synthesized in the liver, but monocytes, macrophages, and dendritic cells also contribute. With a molecular weight of approximately 29 kDa, FHR-2 closely resembles Complement Factor H (CFH), a key regulator of the complement system. Although FHR-2 shares structural similarities with Complement Factor H (CFH), its precise function is still unclear.

Research indicates that FHR-2 may inhibit the alternative pathway (AP) C3 convertase, potentially reducing membrane attack complex (MAC) formation. Conversely, some studies indicate that FHR-2 might instead promote AP activation. Findings support this, as a hybrid mutant protein with FHR-5 domains and full-length FHR-2 lead to overactivation of the complement system.

FHR-2 is linked to various diseases driven by complement dysregulation, such as atypical hemolytic uremic syndrome (aHUS) and age-related macular degeneration (AMD). Its involvement in the alternative pathway makes it a promising therapeutic target for managing complement-driven diseases.

Human FHR-2 can be measured with our HK3004 assay, designed to precisely quantify this intriguing glycoprotein.

Application

Immuno assays

Use

The human FHR-2 ELISA kit is to be used for the in vitro quantitative determination of FHR-2 in serum and plasma samples.

Principle

The human FHR-2 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 1 hour and 15 minutes. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human FHR-2. Peroxidase-conjugated antibody will bind to the captured FHR-2. Peroxidase-conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human FHR-2 standards (log). The human FHR-2 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

Specifications
Downloads

Amount

1 x 96 det., 2 x 96 det.

Product type

Assays

Working volume

100 µl/well

Species

Human

Cross reactivity

Monkey – Yes, Mouse – No, Pig – No, Rat – No

Alias

Complement Factor H-related protein 2

Disease

Autoimmunity, Infectious diseases, Nephrology, Neurological disorders, Tumor immunology

Storage and stability

Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.

Precautions

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.

Assay Manual