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Catalog # HM5011

Prion PrPSC, mAb 1.5D7

125.00430.00

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The monoclonal antibody 1.5D7 recognizes the disease associated isoform of the prion protein
termed PrPSc. Prion diseases, also known as spongiform encephalopathies, are a group of
neurodegenerative diseases that include BSE (bovine spongiform encephalopathy) in cattle, scrapie
in sheep and CJD (Creutzfeldt-Jakob disease) in humans. The normal cellular form of the prion
protein is denoted as PrPC and is a constitutively expressed glycosylphosphatidylinositol anchored
protein that has been shown to play a role in myelin formation. PrPC has an unstructured N-terminal
part and a C-terminal part consisting of three α -helices and two short β strands. Refolding of the
normal prion protein results in PrPSc, which has a tightly packed C-terminal part enriched in beta
sheets which is insoluble and resistant to digestion by proteases. Prion diseases are characterized by
the deposition of highly structured aggregates of PrPSc, astrocytosis, neuronal cell death and
spongiform structures in the brain. These diseases can be sporadically (unknown cause), be inherited
due to polymorphisms or mutations in the prion protein gene or be transmitted by an infectious particle
which is believed to consist of PrPSc only. In order to study prion diseases the detection of PrPSc and
the ability to discriminate between the normal and disease associated PrP is of pivotal importance.
The monoclonal antibody 1.5D7 can be used for the specific identification and characterization of
PrPSc in tissue sections by immunohistochemistry and PET-blot.

Application
Frozen sections, Immuno assays, Paraffin sections, Western blot

Application Notes
W: A reduced sample treatment and SDS-Page was used. The band size is 30 kDa (Ref.1).
IHC-P: Paraffin sections were deparaffinized, rehydrated and endogenous peroxidase was quenched using 0.3% H2O2 in methanol for 20 min. As positive control BSE infected brain tissue was used and as negative control non-diseased brain tissue (Ref.1).

Use
For immunohistochemistry, and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Positive Control
Prion diseased Brain tissue-

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg
Formulation
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Immunogen
BoPrP153-165 (GSDYEDRYYRENM)
Isotype
Mouse IgG2b
Species
N/A
Cross reactivity
Bovine – Yes, Human – Yes, Mouse – Yes
Alias
Spongiform encephalopathies
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Neurological disorders

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